The "scissors" of the molecular biology are: DNA Restriction enzymes.
A restriction enzyme is a protein capable of cleaving a DNA fragment at a characteristic nucleotide sequence called a restriction site. Each restriction enzyme thus recognizes a specific site. Several hundred restriction enzymes are currently known.
Naturally present in a large number of species of bacteria, these enzymes have become important tools in genetic engineering.
The "glue" of the molecular biology are: DNA ligase
In molecular biology, DNA ligases are ligase-class enzymes that catalyze the formation of a phosphodiester bond between two segments of DNA. DNA ligases are involved in several essential cellular processes of DNA metabolism: in DNA replication, suture of Okazaki fragments, and in DNA repair and homologous recombination.
The use of these tools in molecular biology: Cloning
Molecular cloning is one of the bases of genetic engineering. It consists of inserting a DNA fragment (called insert) in an appropriate vector such as a plasmid for example. The new plasmid thus created will then be introduced into a host cell, generally the Escherichia coli bacterium. This will then be selected and multiplied to obtain a large amount of the plasmid of interest. Cloning a gene involves inserting it into a plasmid. A clone will be the bacterial transformant that contains this particular plasmid. In this case we speak of clone because all the individuals of the bacterial colony are genetically identical. Molecular cloning is thus different from reproductive cloning (creating an individual genetically identical to another but of a different age) or therapeutic cloning (making tissues from stem cells to perform transplants compatible with the recipient).
Molecular cloning requires restriction enzymes capable of cleaving the DNA, and DNA ligase capable of re-gluing the DNA fragments. Ligase was isolated for the first time from T4 bacteriophage. This enzyme is involved in the repair and replication of DNA. It can bind DNA fragments with compatible sticky ends. At higher concentration, this enzyme is also able to bind two ends of DNA as shown here. T4 DNA ligase works using ATP and Mg ++. It has an activity optimum of 16 ° C, but remains active at room temperature.
Proteins that are functionally less important for the survival of an organism generally evolve faster than more important proteins.
Proteins serve as the building blocks for all of life's essential processes. The proteins evolve along with the genes that create them, adding new functionality or features that may potentially result in the development of new species.
The mutation of amino acid-coding nucleotides and the stabilization of novel variations in the population are the two phases required for protein evolution.
The stability of a protein's folded structure, how well it prevents aggregation, and how well it is chaperoned all affect how quickly it evolves. According to the studies, the degree of a protein's expression has a greater influence on its evolutionary rate than does the protein's functional significance.
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Answer:
D. Red, Orange, Yellow, Green, Blue, Indago, Violet.
Explanation:
Violet has the highest freqency and red has the lowest.
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Answer:
mitosis is a process of cell division in which one cell is divided into two and the two cells produced are exacty similar to the parent cell. chromosome number cell size remains same.
mitosis plays an important role in growth of organisms.
Explanation:
