Answer:
c.) loss of chloroplasts
Explanation:
Chloroplasts are the double membrane-bound organelles of photosynthetic eukaryotes and serve as the site for photosynthesis. The organisms that can carry out photosynthesis make the organic nutrients and do not depend on other organisms for food.
According to the given information, the photosynthetic protists become parasite in fish and start deriving nutrition from the host. If the protists continue to survive as a parasite to fish, the chloroplasts will be rendered non-significant. The parasitic mode of nutrition does not require chloroplasts and therefore, the protists would lose the organelles over generations.
This is because there might not be one restriction site bordering the gene to be cloned and the identical restriction site in the plasmid. Since these two restriction enzymes make compatible sticky ends, the insert has a chance of combining with the plasmid. To ensure efficient digestion, the two recognition sites should be more than 10 base pairs apart. If one of the enzymes is a poor cutter or if the sites are separated 10 base pairs or less, the digestions <span>should be performed sequentially</span>
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