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ehidna [41]
2 years ago
11

Who wnata to date over snap we show what ever we want I mean what ever im 13, 14 is the oldest I can go.

Chemistry
1 answer:
iren [92.7K]2 years ago
4 0

Answer: I already gave u the aNswer

Explanation:0  ok bye

bye .................................

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The breaking buffer that we use this week contains 10mM Tris, pH 8.0, 150mM NaCl. The elution buffer is breaking buffer that als
olasank [31]

Answer:

A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula M_1*V_1 = M_2*V_2. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL)  with water

Explanation:

Given data includes:

Tris= 10mM

pH = 8.0

NaCl = 150 mM

Imidazole = 300 mM

In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.

Stock Concentration             Volume to be             Final Concentration

                                               added            

1 M Tris                                     2.5 mL                         10 mM

5 M NaCl                                  7.5 mL                        150 mM

1 M Imidazole                           75 mL                         300 mM    

M_1*V_1 = M_2*V_2. is the formula that is used to determine the corresponding volume that is added for each stock concentration

The stock concentration of Tris ( 1 M ) is as follows:

M_1*V_1 = M_2*V_2.

1*V_1= 0.01 M *250mL\\V_1 = 2.5mL

The stock concentration of NaCl (5 M ) is as follows:

M_1*V_1 = M_2*V_2.

1*V_1= 0.15 M *250mL\\V_1 = 7.5mL

The stock concentration of Imidazole (1 M ) is as follows:

M_1*V_1 = M_2*V_2.

1*V_1= 0.03 M *250mL\\V_1 = 75mL

Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.

5 0
3 years ago
A first-order reaction is 81omplete in 264s. A. What is the half-life for this reaction? b. How long will it take for the reacti
Arlecino [84]

A first-order reaction is 81omplete in 264s.The half-life for this reaction (i) t 1/2 = =3.465×10 −3 s.to reach 95% Completion = 285 s.

To measure reaction rates, chemists initiate the reaction, measure the concentration of the reactant or product at different times as the reaction progresses,

For a 0-order response, the mathematical expression that may be employed to determine the half of life is: t1/2 = [R]0/2k. For a first-order reaction, the half of-existence is given by: t1/2 = zero.693/ok. For a 2d-order response, the method for the half-life of the response is: 1/okay[R]0

The 1/2-life of a response (t1/2), is the quantity of time needed for a reactant concentration to lower via half of compared to its initial awareness. Its software is used in chemistry and medicine to are expecting the awareness of a substance over time

Half of the lifestyles is the time required for exactly 1/2 of the entities to decay   50%.

Learn more about first order reaction here:-

#SPJ4

5 0
1 year ago
An ionic bond can be formed when one or more electrons are:
Mashutka [201]
D - for example, Potassium has 1 electron on its outer shell, whilst Chlorine has 7 electrons on its outer shell. Potassium loses one electron to Chlorine so that each of them have a full outer shell. This would form Potassium Chloride.
6 0
3 years ago
Does anyone know what Hydrogen's oxidation state would be if it was acting as an anion non-metal?
Ilia_Sergeevich [38]

Answer:

yeah,The oxidation state of an atom does not represent the "real" charge on that atom, or any other actual atomic property.Hydrogen has OS = +1, but adopts −1 when bonded as a hydride to metals or metalloids. Oxygen in compounds has OS = −2. This set of postulates covers .

Explanation:

3 0
3 years ago
Which graphic below shows the correct orientation of each water molecule when it is near the cl- ion in the aqueous solution?
andrew-mc [135]
The question is missing the graphics required to answer which I have attached as an image.

There are four different representations of the orientation of water molecules around chloride anion. Let's first analyze the water molecule.

We have H-O-H as the structure of water. The oxygen atom is more electronegative than the hydrogen atoms, which results in a partial positive charge on the hydrogen atoms and a partial negative charge on the oxygen atom.

The chloride anion is a negative charge. Therefore, the water molecules should orient themselves with the hydrogen atoms facing the chlorine atom as the partial positive charge on the hydrogen atoms will be attracted to the negative charge of the chlorine atom.

The correct representation is shown in graph 3 which shows all hydrogen atoms facing the chlorine anion.

8 0
3 years ago
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