1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
ivanzaharov [21]
3 years ago
15

The breaking buffer that we use this week contains 10mM Tris, pH 8.0, 150mM NaCl. The elution buffer is breaking buffer that als

o contains 300mM imidazole. Describe how the instructor made the 0.25L elution buffer for all the students this week given 500ml of 1M of Tris (121.1 g/mole) (pH8.0), 750ml of 5M NaCl (MW = 58.44 g/mole) and 350ml of 1M imidazole (68.08 g/mole). Show your calculations for credit.
Chemistry
1 answer:
olasank [31]3 years ago
5 0

Answer:

A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula M_1*V_1 = M_2*V_2. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL)  with water

Explanation:

Given data includes:

Tris= 10mM

pH = 8.0

NaCl = 150 mM

Imidazole = 300 mM

In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.

Stock Concentration             Volume to be             Final Concentration

                                               added            

1 M Tris                                     2.5 mL                         10 mM

5 M NaCl                                  7.5 mL                        150 mM

1 M Imidazole                           75 mL                         300 mM    

M_1*V_1 = M_2*V_2. is the formula that is used to determine the corresponding volume that is added for each stock concentration

The stock concentration of Tris ( 1 M ) is as follows:

M_1*V_1 = M_2*V_2.

1*V_1= 0.01 M *250mL\\V_1 = 2.5mL

The stock concentration of NaCl (5 M ) is as follows:

M_1*V_1 = M_2*V_2.

1*V_1= 0.15 M *250mL\\V_1 = 7.5mL

The stock concentration of Imidazole (1 M ) is as follows:

M_1*V_1 = M_2*V_2.

1*V_1= 0.03 M *250mL\\V_1 = 75mL

Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.

You might be interested in
Need help answering
svp [43]

Answer:

Your answer would be E

Explanation:

4 0
2 years ago
Smartphone batteries contain a casing that surrounds it that is made up out of aluminium. What would best describe the propertie
Vladimir79 [104]

Answer:

it contains the electricity of the battery

Explanation:

6 0
2 years ago
Why are you so much older on Mercury?
Alinara [238K]

Answer:As you can see from the very different numbers in the boxes above, your age changes (sometimes quite a lot) ... Mercury is the closest planet to the Sun and so has a smaller orbit path – it takes just 88 Earth days

Explanation:

4 0
3 years ago
Read 2 more answers
Elena makes the table below to determine the number of atoms of each element in the chemical formula 3(NH4)2SO4.
Ymorist [56]

Answer:

plqojs then came back and her story about how the ocean had become toper for her own purposes and share the ocean and the mosquitoes and it that way in notebook life that they were never able of English orto make the ocean view the mosquitoes in the first place they would have to make it to.

5 0
3 years ago
Read 2 more answers
What is displacement?
mars1129 [50]
Displacement reaction is a chemical reaction in which a more reactive element displaces a less reactive element from its compound. Both metals and non-metals take part in displacement reactions. Example : Reaction of iron nails with copper sulphate solution.
8 0
3 years ago
Other questions:
  • 1. identify whether following have ionic or covalent bonding. Give your reasons: Oxygen molecule, water, calcium oxide, hydrogen
    6·1 answer
  • Which property of waves is the distance between one point on a wave and that same point on the wave next to it?
    10·2 answers
  • Write the balanced molecular and net ionic equations for the reaction between aluminum metal and silver nitrate. identify the ox
    8·2 answers
  • The "normal" lead content in human blood is about 0.40 part per million (i.e., 0.40 g of lead per million grams of blood). A val
    14·2 answers
  • The is the rate which a substance undergoes a chemical change
    5·1 answer
  • Roberto and his dad drove from town A to town B taking the long way, ate lunch and then came back using a
    6·2 answers
  • What is used to contain an acid spill and what are the characteristics of them?​
    8·1 answer
  • = 25 X 5 = (use the correct number of sig figs)
    13·1 answer
  • 2.50 grams F2= _____ grams of F2
    15·1 answer
  • A 400. 0 g sample of liquid water is at 30. 0 ºc. how many joules of energy are required to raise the temperature of the water t
    14·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!