Answer:
C. Trp D. Phe E. Tyr
Explanation:
The concentration of a protein has a direct relation with absorbance of the protein in a UV spectrophotometer. The formula which relates concentration with absorbance is described as under:
A = ∈ x c x l
where, A = Absorbance
∈ = Molar extinction co-efficient
c = Concentration of absorbing species i.e. protein
l = Path length of light
Tryptophan (Trp), phenylalanine (Phe ) and tyrosine (Tyr) are three aromatic amino acids which are used to measure protein concentration by UV. It is mainly because of tryptophan (Trp), protein absorbs at 280 nm which gives us an idea of protein concentration during UV spectroscopy.
The table depicting the wavelength at which these amino acids absorb and their respective molar extinction coefficient is as under:
Amino acid Wavelength Molar extinction co-efficient (∈)
Tryptophan 282 nm 5690
Tyrosine 274 nm 1280
Phenylalanine 257 nm 570
In view of table above, we can easily see that Molar extinction co-efficient (∈) of Tryptophan is highest amongst all these 3 amino acids that is why it dominates while measuring concentration.
Answer:
0.00559 mol
Explanation:
The following data were obtained from the question:
V = 140mL = 0.14L
T = 296K
P = 98.2kPa = 98200Pa
Recall: 101325Pa = 1atm
98200Pa = 98200/101325 = 0.97atm
R = 0.082atm.L/Kmol
n =?
The number of mole (n) can be obtained as follows:
PV = nRT
n = PV /RT
n = (0.97x0.14)/(0.082x296)
n = 0.00559 mol
Therefore, the number of mole of the gas present is 0.00559 mol
Answer:
Sound waves required a medium.
Explanation:
In space, there is only vacuum hence no any medium for sound wave travel.
Since sound waves are mechanical waves, they require a material medium such as air, hence no sound transmission.
Answer:
Bottle 2 shows the vinegar has a molarity of 2M acetic acid
Explanation:
2M of acetic acid has higher concentration of acetic acid as compared to other vinegar. In 2M of acetic acid is equals to 120 gram of acetic acid in 500 ml of water which is quiet higher than other concentration of acetic acid in vinegar which is 4%. So Mr. Holmes has to use 2M of acetic acid which will produced high intense eruption in the volcano and win the national science fair contest.
Answer:
Element X has valency 2 while Y Valency 2, element Y has high tendency of gaining electrons for its stability thus the compound formed will be B
elements X is positively charged while Y will be negatively charged
