In the experiment with plants being grown at different temperatures to see how it will affect a plant's growth, the control group is the plant being grown at room temperature. The experimental group includes the three plants being grown at temperatures greater than room temperature.
For obtaining an adequate amount of DNA samples for DNA fingerprinting, polymerase chain reaction (PCR) can be used.
Polymerase chain reaction (PCR) is a technique that is used to amplify the DNA sample. The steps included in PCR are:-
1. Denaturation of the double-stranded DNA (at around 95°C)
2. Annealing of the primers to each of the single-stranded DNA templates ( at around 65°C)
3. Extension or elongation of the primers by considering every single-stranded DNA as a template. This elongation is carried out by <em>Taq </em>polymerase (at around 72°C).
With each cycle of PCR, the DNA sample is increased by 2ⁿ. Here, 'n' represents the number of PCR cycles.
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They leave their stoma closed during the day and opened during the night. Hope this helps.