Restriction enzymes can also be used to generate compatible ends on PCR products. In all cases, one or more restriction enzymes are used to digest the DNA resulting in either non-directional or directional insertion into the compatible plasmid. The most common Type II enzymes are those like HhaI (NEB #R0139), HindIII (NEB #R0104), and NotI (NEB #R0189)
Energy. A solute can only move against concentration gradients using active transport which requires energy
<span>Increase in soil erosion. hop it helps.</span>
A strong incentive affects the Kendall's hunger drive.
<u>Explanation:</u>
Kendall was waiting in a line at a local coffee shop. She already had her breakfast for the day. The aroma of freshly baked bagels and muffins began to increase her hunger. Kendall faced a strong incentive affecting the hunger drive.
Imbalance of hormones like leptin and serotonin causes food cravings. After food consumption, endorphins gets released into the body. This is the reason for food craving and sometimes leads to addiction.