Increasing substrate concentration also increases the rate of reaction to a certain point. Once all of the enzymes have bound, any substrate increase will have no effect on the rate of reaction, as the available enzymes will be saturated and working at their maximum rate.
it states that the atom is neutral, meaning the number of electrons and protons are the same. so if there are 13 electrons, there are 13 protons. And the mass number is neutrons plus protons. So 13+15 is 28