0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
<h3>What is dilution?</h3>
Dilution is a process of making a solution of lower concentration from a solution of higher concentration by the addition of solvent to a given volume of the solution of higher concentration.
Dilution of solutions is done using the dilution formula in order to determine the given volume of diluent or stock solution required. The dilution formula is given below:
where:
- C1 = Initial concentration of enzyme
- C2 = Final concentration of enzyme
- V1 = Initial volume
- V2 = Final volume
For the enzyme dilution;
C1 = 1 mg/mL
C2 = 1/50 mg/ml = 0.02 mg/ml
V= ?
V2 = 5 ml
V1 = C2V2/C1
V1 = 0.02 * 5/1 = 0.1 mL
Therefore, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
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Answer:
Explanation:
When potassium chloride reacts with lead acetate in aqueous medium, it gives white precipitate of lead chloride and potassium acetate in aqueous medium as a product.
As shown in chemical reaction given below:
Answer:
-1815.4 kJ/mol
Explanation:
Starting with standard enthalpies of formation you can calculate the standard enthalpy for the reaction doing this simple calculation:
∑ n *ΔH formation (products) - ∑ n *ΔH formation (reagents)
This is possible because enthalpy is state function meaning it only deppends on the initial and final state of the system (That's why is also possible to "mix" reactions with Hess Law to determine the enthalpy of a new reaction). Also the enthalpy of formation is the heat required to form the compound from pure elements, then products are just atoms of reagents organized in a different form.
In this case:
ΔH rxn = [(2 * -1675.7) - (3 * -520.0)] kJ/mol = -1815.4 kJ/mol
