The amino acids in the original partial sequence (N-term-...P-I-E...-C-term) have little to no impact on protein structure and f
unction. However the amino acid sequence near the C-terminal end of the protein is required for normal function. Any amino acid changes in this region of the protein will result in a nonfunctional protein. Given this information, which reversion mutation in the gene would restore the normal function of the protein
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The water would come out the bucket in one direction which is downwards