What can happen if the level of the developing liquid is higher than the applied spots in the tlc analysis?

1 answer:

7 0

If the solvent level in the developing jar is deeper than the origin (spotting line) of the TLC plate, the solvent will dissolve the compounds into the solvent reservoir instead of allowing them to move up the plate by capillary action. Thus, you will not see spots after the plate is developed.

What is Thin Layer Chromatography (TLC)?

TLC is a simple, quick, and inexpensive procedure that gives the chemist a quick answer as to how many components are in a mixture.
TLC is also used to support the identity of a compound in a mixture when the Rf of a compound is compared with the Rf of a known compound.
A TLC plate is a sheet of glass, metal, or plastic which is coated with a thin layer of a solid adsorbent (usually silica or alumina). A small amount of the mixture to be analysed is spotted near the bottom of this plate.
The TLC plate is then placed in a shallow pool of a solvent in a developing chamber so that only the very bottom of the plate is in the liquid. This liquid, or the eluent, is the mobile phase, and it slowly rises up the TLC plate by capillary action.
As the solvent moves past the spot that was applied, an equilibrium is established for each component of the mixture between the molecules of that component which are adsorbed on the solid and the molecules which are in solution.
In principle, the components will differ in solubility and in the strength of their adsorption to the adsorbent and some components will be carried farther up the plate than others. When the solvent has reached the top of the plate, the plate is removed from the developing chamber, dried, and the separated components of the mixture are visualized. If the compounds are coloured, visualization is straightforward.
Usually the compounds are not coloured, so a UV lamp is used to visualize the plates. The plate itself contains a fluorescent dye which glows everywhere except where an organic compound is on the plate.

To learn more about TLC: brainly.com/question/13483325

#SPJ4

You might be interested in

What happens during jury deliberation in a criminal court case?

dlinn [17]

The following steps are usually followed: The foreperson tells the court security officer that a verdict has been reached. The judge calls everyone, including you, back into the courtroom, The clerk in the courtroom asks the foreperson for the verdict. The verdict is read into the record in open court.

4 0

3 years ago

When iron reacts with oxygen what substance is produced?

zhannawk [14.2K]

It will produce iron oxide

8 0

3 years ago

Calculate the net force 175 N 1125 N

Ket [755]

Answer:

<h3>_____________&_________</h3><h2>Net force = sum of all forces with directions</h2><h2>so.....</h2><h2>NET FORCE = 125+(-75)</h2><h2> = 50 N</h2><h2> in the direction of yellow </h2><h2> t-shirt man</h2><h2>------------------------------------</h2>

4 0

3 years ago

In an experiment, mice were fed glucose (C₆H₁₂O₆) containing a small amount of radioactive carbon. The mice were closely monitor

Luden [163]

<h3>Answer:</h3>

Carbon dioxide

Explanation:

Cellular respiration involves a break down of sugars such as glucose to yield energy in the form of ATP and water together with carbon dioxide as byproducts.
In this case, when mice are fed with glucose, cellular respiration takes place breaking down glucose to yield energy in the form of ATP, water and carbon dioxide.
Therefore, the radioactive carbon in glucose showed up in carbon dioxide, since the carbon in glucose ends up in carbon dioxide.

8 0

3 years ago

What is the difference between 0.50 mol HCl and 0.50 M HCl?

iVinArrow [24]

Answer:

Here you go

Explanation:

6 0

3 years ago