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Anastaziya [24]
2 years ago
6

What can happen if the level of the developing liquid is higher than the applied spots in the tlc analysis?

Chemistry
1 answer:
Alexeev081 [22]2 years ago
7 0

If the solvent level in the developing jar is deeper than the origin (spotting line) of the TLC plate, the solvent will dissolve the compounds into the solvent reservoir instead of allowing them to move up the plate by capillary action. Thus, you will not see spots after the plate is developed.

What is Thin Layer Chromatography (TLC)?

  • TLC is a simple, quick, and inexpensive procedure that gives the chemist a quick answer as to how many components are in a mixture.
  • TLC is also used to support the identity of a compound in a mixture when the Rf of a compound is compared with the Rf of a known compound.
  • A TLC plate is a sheet of glass, metal, or plastic which is coated with a thin layer of a solid adsorbent (usually silica or alumina). A small amount of the mixture to be analysed is spotted near the bottom of this plate.
  • The TLC plate is then placed in a shallow pool of a solvent in a developing chamber so that only the very bottom of the plate is in the liquid. This liquid, or the eluent, is the mobile phase, and it slowly rises up the TLC plate by capillary action.
  • As the solvent moves past the spot that was applied, an equilibrium is established for each component of the mixture between the molecules of that component which are adsorbed on the solid and the molecules which are in solution.
  • In principle, the components will differ in solubility and in the strength of their adsorption to the adsorbent and some components will be carried farther up the plate than others. When the solvent has reached the top of the plate, the plate is removed from the developing chamber, dried, and the separated components of the mixture are visualized. If the compounds are coloured, visualization is straightforward.
  • Usually the compounds are not coloured, so a UV lamp is used to visualize the plates. The plate itself contains a fluorescent dye which glows everywhere except where an organic compound is on the plate.

To learn more about TLC: brainly.com/question/13483325

#SPJ4

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Answer:

Uses nuclear reactions to produce energy

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Explanation:

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During the process of laser fusion, small pellets of deuterium-tritium (DT) isotopes mixture are fed into a blast chamber where they are compressed to high densities using a number of amplified laser beams in the chamber.

The high energy density of the beams as well as the heat produced due to compression, induces the thermonuclear explosion ignition resulting in the production of high energetic products such as charged particles, x-rays and neutrons. The energy produced is absorbed and stored as heat in a blanket that is then used in a steam thermal cycle to generate electrical power.

There are two methods of compression of the DT pellet: direct and indirect-drive laser fusions.

However, there are a number of limitations to energy production by this process. One limitation is that the process is extremely inefficient in energy energy production. Also, the heat produced by the flashtubes results innthe deformation of the laser glass.

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How much heat is needed to raise the temperature of 55.0 g sample of water by 65.0 oC.
sveticcg [70]

Answer: 14943.5 J

Explanation:

The quantity of heat energy (Q) required to raise the temperature of a substance depends on its Mass (M), specific heat capacity (C) and change in temperature (Φ)

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Q = ?

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Then, Q = MCΦ

Q = 55.0g x 4.18 J/g°C x 65.0°C

Q = 14943.5 J

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Answer: 226 Subscript 88 Baseline Upper R a right arrow Superscript 222 Subscript 86 Baseline Upper R n + Superscript 4 Subscript 2 Baseline Upper H e

Explanation:

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The general representation of alpha decay reaction is:

^{A}_{Z}\textrm{X}\rightarrow ^{A-4}_{Z-2}\textrm {Rn}+ ^{4}_{2}\textrm{He}

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