Question

What is mixed-acid fermentation? how is methyl red used to determine if a microbe has undergone mixed-acid fermentation?

Answer 1

Mixed Acid Fermentation is a biological process by which a six carbon sugar is converted into complex mixture of acids. It is an example of anaerobic fermentation reaction which is commonly seen in bacteria.

The fermentation produces lactate, acetate, succinate, formate, ethanol, $H_{2}$ and $CO_{2}$ and their formation depends upon the presence of certain key enzymes in the bacterium.

The Methyl Red test is used in detecting occurring of mixed acid fermentation pathway when glucose is provided to microbes by using ph indicator. If the fermentation pathway has taken place, the mixture of acids will make the solution very acidic and causes red color change. The Methyl Red test belongs to the group called as IMViC tests.

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