The ddNTP lacks the oxygen atom at 3' carbon in addition to at the 2'carbon which is lacking in the dNTP.
<h3>What is Sanger sequencing? </h3>
It is a chain termination method developed by Frederick Sanger and colleagues in 1977.
The process to determine the sequence of nucleotide bases in a part of a DNA molecule is called DNA sequencing. In the process of Sanger sequencing, the DNA molecule to be sequenced is copied multiple times by using polymerase chain reaction (PCR).
This creates DNA fragments of different lengths. Fluorescent labelled dideoxynucleotides are then used to terminate further chain formation.
This process marks the end of fragments and allows sequence determination. Therefore, a ddNTP is used in the Sanger Sequencing process. Compared to the normal DNA precursors, ddNTP lacks oxygen at the 3' carbon.
Read more about Sanger sequencing, here
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