Step One - Obtain a clean microscope slide.
Step Two - Place a drop of liquid on the slide. This is the “wet” part of the wet mount. The liquid used depends on the type of cell being viewed:
If examining a plant cell, tap water can be used.
If examining an animal cell, physiological saline (or contact lens solution) must be used, because if plain water is used, the cell will explode from osmotic pressure. Unlike plant cells and bacteria, animal cells have no cell wall to structurally support them.
Step Three - Obtain the specimen to be used. Some introductory biology classics for viewing include:
Skin of an onion bulb: In order to view the cells, a very thin layer of skin must be obtained. Take a single layer of onion and bend it towards the shiny side. After it snaps, pull gently, and a transparent layer of skin, similar to Scotch tape, will appear.
Elodea leaf: Elodea leaves are two cell layers thick. The cells in one layer are smaller than the cells in the other, so elodea leaves can be used to better understand a microscope's depth of field.
Cheek cells: Human epithelial cells can be obtained by gently rubbing a toothpick on the inside of the mouth, and then swirling the toothpick in the physiological saline on the slide.
Pond water: Obtaining some water from a pond makes wet mount preparation a breeze, since the water and the specimens are both included.
Hope this helps
Answer: Aldolase
Explanation:
In the metabolism of glucose( glycolysis) phosphofructokinase is an enzyme that catalyzes the conversation of fructose-6-phosphate into fructose-1,6-bisphosphate. This in turn is converted to pyruvate after various steps of enzymatic activity in the glycolytic pathway.
If phosphofructokinase experienced a mutation that interfered with substrate binding, the enzyme that is going to be most immediately impacted in terms of accessing substrate is the ALDOLASE.
Aldolase enzymes cleave fructose-1,6-bisphosphate to triose phosphates( glyceraldehyde 3-phosphate and dihydroxy-acetone phosphate) facilitating an increase in anaerobic production of ATP in muscle.
Therefore, the substrate for binding of aldolase, which is fructose-1,6-bisphosphate is lacking due to mutation of phosphofructokinase enzyme.
Experimentation to determine the temperature of stars
Answer:
36 ATPs
Explanation:
Cellular respiration starts with glycolysis wherein glucose is broken down into two molecules of pyruvate. The process of glycolysis forms two molecules of ATP by substrate-level phosphorylation for each glucose molecule. Both pyruvate molecules are converted into acetyl CoA to enter into the Kreb's cycle. Kreb's cycle forms two ATP molecules by substrate-level phosphorylation. NADH and FADH2 formed during glycolysis and Kreb's cycle are oxidized by the electron transport chain. This process also forms as many as 34 ATP molecules. If acetyl CoA is not formed, the total ATP gain per glucose will be only 2 ATP molecules (from glycolysis only) which is 38-2= 36 less than the total.
Answer:
Digestion, distribution of nutrients throughout the body, and it can serve as a hydrostatic skeleton.