Phase contrast microscope shows cells against a bright background and the intracellular structures of unstained cells based on their varying densities.
A technique called phase-contrast microscopy is used to contrast a translucent object without staining it. Phase-contrast microscopy has a number of benefits, including the ability to employ high-resolution objectives, but doing so necessitates a specialised condenser and more expensive objectives.
With the help of the optical contrast technique known as phase contrast, biological specimens' cells can show their unstained architecture. With brightfield illumination, cell structures that appear transparent can be seen in high contrast and in great detail with phase contrast.
Phase-contrast photographs often have a grey backdrop with varying amounts of light and dark elements. Halo-light ring creation, a drawback of phase-contrast microscopy, is one.
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