1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
In-s [12.5K]
1 year ago
8

Name three methods of disrupting cells before fractionation

Biology
1 answer:
sladkih [1.3K]1 year ago
4 0

Extraction, homogenization, and centrifugation are the three processes in cell fractionation.

Cell fractionation is a method for dividing cellular components while yet maintaining each component's unique functionality. This technique was initially applied to show where distinct biochemical activities are located within cells. Subcellular fractionation is also used to help with illness detection and to offer an enhanced source of protein for further purification. To prevent osmotic damage, tissue is routinely homogenized in an isotonic buffer solution. Grinding, mincing, chopping, pressure changes, osmotic shock, freeze-thawing, and ultrasonic waves are a few of the mechanisms for homogenization. After that, the samples are maintained cool to avoid enzymatic deterioration. It is the development of a uniform mass of cells (cell homogenate or cell suspension).

Learn more about  Subcellular fractionation here:-

brainly.com/question/11076837

#SPJ1

You might be interested in
During cellular respiration the largest amount of ATP is generated in
Degger [83]
The electron transport chain of the cellular respiration process produces maximum ATP.

There are three parts of cellular respiration:

Glycolysis, which produces 2 ATP.
Krebs cycle, which does not produce any ATP. It produces only the raw materials for ATP production.
Oxidative phosphorylation, which involves the electron transport chain that produces maximum ATP. It takes one NADH molecule via Channel 1 to produce 2.5 ATP and one FADH2 molecule via channel 2 to produce 1.5 ATP, for a total of 34 ATP.
3 0
3 years ago
Rods are to cones as _____ is(are) to _____. peripheral vision and night vision; color vision and visual acuity color vision and
VashaNatasha [74]

The answer would be peripheral vision and night vision; color vision and visual acuity. Peripheral vision is the effort of the rods, nerve cells situated mainly external the macula (the midpoint) of the retina. The rods are also accountable for night vision and low-light vision but are unresponsive to color. Visual acuity is the sharpness of vision. Color vision examinations check your capability to differentiate colors.

3 0
3 years ago
A raccoon consumes 500 calories of food. 50 of those
exis [7]

Answer:

10 % of the energy (i.e., 50 calories) is converted to biomass . This efficiency of energy transformation is high

4 0
3 years ago
Where are fats, carbohydrates, and proteins broken down into particles small enough to be absorbed into the body?
siniylev [52]

Answer:

Duodenum (option 3)

Explanation:

Digestion of carbohydrates, proteins, and fats continues in the small intestine. Starch and glycogen are broken down into maltose by small intestine enzymes. Proteases are enzymes secreted by the pancreas that continue the breakdown of protein into small peptide fragments and amino acids.

The duodenum is the first section of the small intestine and is the shortest part of the small intestine. It is where most chemical digestion using enzymes takes place

8 0
3 years ago
Read 2 more answers
Which factors can have the greatest effect on the health of a river
Tomtit [17]
It’s the letter C.................
5 0
3 years ago
Other questions:
  • The DNA bases arrange themselves in A) every possible combination b) combinations that depend on certain shapes
    11·1 answer
  • Eosinophils function in Eosinophils function in production of heparin. production of surface antigens for red blood cells. blood
    14·1 answer
  • The _________ run(s) adjacent to the medial surface of the upper limb and eventually helps form the axillary vein.
    15·2 answers
  • Triglycerides and cholesterol do not circulate freely in the bloodstream. True or False
    8·1 answer
  • What process causes your feet to feel hot when you walk on a hot surface
    8·1 answer
  • During the production of fertilizer, NH(am) reacts with H3PO4(aq). For this chemical reaction, match each description with the a
    12·2 answers
  • Bryophytes exhibit sexual reproduction by producing male and female gametes which structure in bryophytes produces the male game
    8·1 answer
  • How is life going for everyone talk to me mines is saf
    10·2 answers
  • How did Durkheim compare society to the human body?
    13·1 answer
  • What type of disorder is sickle cell anemia
    11·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!