What is the purpose of adding 0. 1% agar to the trypticase nitrate broth when conducting the nitrate reduction test?.

Chemistry

1 answer:

PilotLPTM [1.2K]1 year ago

5 0

Agar is used to assist establish an anaerobic environment that promotes nitrate reduction.

Nitrate Reduction test:

The nitrate in the broth is converted to nitrite by organisms that can produce the nitrate reductase enzyme, which can then be further converted to nitric oxide, nitrous oxide, or nitrogen.
Anaerobic respiration and denitrification are two processes that can convert nitrate to a variety of compounds.
While denitrification only reduces nitrate to molecular nitrogen, anaerobic respiration employs nitrate as the bacterium's final electron acceptor, reducing it to a range of chemicals.
The nitrate reduction test is based on the detection of nitrite and its capacity to produce a red precipitate (prontosil), which is a water-soluble azo dye, when it combines with sulfanilic acid to create a complex (nitrite-sulfanilic acid).

Learn more about the Nitrate reduction test with the help of the given link:

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if you are told to get 100 mL of stock solution to use to prepare smaller size sample for an experiment, which piece of glasswar

fgiga [73]

Answer:

A beaker

Step-by-step explanation:

Specifically, I would use a 250 mL graduated beaker.

A beaker is appropriate to measure 100 mL of stock solution, because it's easy to pour into itscwide mouth from a large stock bottle.

You don't need precisely 100 mL solution.

If the beaker is graduated, you can easily measure 100 mL of the stock solution.

Even if it isn't graduated, 100 mL is just under half the volume of the beaker, and that should be good enough for your purposes (you will be using more precise measuring tools during the experiment).