All categories

9 months ago

What is the purpose of adding 0. 1% agar to the trypticase nitrate broth when conducting the nitrate reduction test?.

Chemistry

1 answer:

PilotLPTM [1.2K]9 months ago

5 0

Agar is used to assist establish an anaerobic environment that promotes nitrate reduction.

Nitrate Reduction test:

The nitrate in the broth is converted to nitrite by organisms that can produce the nitrate reductase enzyme, which can then be further converted to nitric oxide, nitrous oxide, or nitrogen.
Anaerobic respiration and denitrification are two processes that can convert nitrate to a variety of compounds.
While denitrification only reduces nitrate to molecular nitrogen, anaerobic respiration employs nitrate as the bacterium's final electron acceptor, reducing it to a range of chemicals.
The nitrate reduction test is based on the detection of nitrite and its capacity to produce a red precipitate (prontosil), which is a water-soluble azo dye, when it combines with sulfanilic acid to create a complex (nitrite-sulfanilic acid).

Learn more about the Nitrate reduction test with the help of the given link:

brainly.com/question/11181586

#SPJ4

You might be interested in

Create a story and describe the motion (in meters) of an object that has traveled a distance but has no displacement. Need Help

vekshin1

Answer:

One complete revolution around a circular path.

Explanation:

Let us take the case of a car moving in a circular track of radius r metres.

In one revolution, the car covers the length(distance) equal to the perimeter of the circle.

In this case, distance traveled = 2 $\pi$ r metres

But after one complete revolution, the car reaches the same position as it was at the beginning of the motion.

Hence, the initial and final points coincide or the car hasn't changed it's position w.r.t the initial point.

So in this case, the displacement is zero.

Hence, revolution of a car around a circular path is an example of an object traveling a distance but having no displacement.

3 0

3 years ago

Consider the following reaction NHAHS(s)NH3(g) + H2S(g) If a flask maintained at 302 K contains 0.196 moles of NH4HS(s) in equil

quester [9]

Answer:

Kc = 3.72 × 10⁶

Explanation:

Let's consider the following reaction:

NH₄HS(g) ⇄ NH₃(g) + H₂S(g)

At equilibrium, we have the following concentrations:

[NH₄HS] = 0.196 M (assuming a 1 L flask)

[NH₃] = 9.56 × 10² M

[H₂S] = 7.62 × 10² M

We can replace this data in the Kc expression.

$Kc=\frac{[NH_{3}] \times [H_{2}S] }{[NH_{4}HS]} =\frac{9.56 \times 10^{2} \times 7.62 \times 10^{2}}{0.196} =3.72 \times 10^{6}$

7 0

2 years ago

The theoretical yield for a reaction is 55.9 g LiCl. The actual yield is 24.6 g LiCl. What is the percent yield of the reaction?

xenn [34]

In chemical reactions, the actual yield is not the same as the expected yield . Actual yield is lower than the theoretical yield . Then we have to find the yield percentage. To see what percentage of the theoretical yield is the actual yield.
Percent yield = actual yield / theoretical yield x 100%
Percent yield = 24.6/55.9 x100%
Percent yield = 44%

7 0

2 years ago

Read 2 more answers

C. Chemical energy. What is the difference in chemical energy between organic and inorganic

Olenka [21]

There are chief differences between organic and inorganic compounds. ... The main difference is in the presence of a carbon atom; organic compounds will contain a carbon atom (and often a hydrogen atom, to form hydrocarbons), while almost all inorganic compounds do not contain either of those two atoms.

8 0

3 years ago

Why Are Bees Important To The Environment/Ecosystem

GaryK [48]

Answer:

They are pollinators of alfalfa.

Explanation:

Without them crops wouldn't be here.

7 0

2 years ago