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Kipish [7]
3 years ago
14

Th e molar absorption coeffi cients of tryptophan and tyrosine at 240 nm are 2.00 × 103 dm3 mol−1 cm−1 and 1.12 × 104 dm3 mol−1

cm−1 , respectively, and at 280 nm they are 5.40 × 103 dm3 mol−1 cm−1 and 1.50 × 103 dm3 mol−1 cm−1 . Th e absorbance of a sample obtained by hydrolysis of a protein was measured in a cell of thickness 1.00 cm and was found to be 0.660 at 240 nm and 0.221 at 280 nm. What are the concentrations of the two amino acids?
Chemistry
1 answer:
nataly862011 [7]3 years ago
4 0

Answer:

5.43x10⁻⁵ dm³mol⁻¹= Concentration of tyrosine

2.58x10⁻⁵ dm³mol⁻¹= Concentration of tryptophan

Explanation:

Lambert-Beer law is:

A = E×C×l

Where A is measured absorbance, E is absorption coefficient, C is concentration of solution and l is optical path length.

For the result at 240nm, it is possible to write:

0.660 = 2.00x10³ dm³mol⁻¹cm⁻¹× Ctry × 1cm + 1.12x10⁴ dm³mol⁻¹cm⁻¹× Ctyr × 1cm <em>(1)</em>

At 280 nm:

0.221 = 5.40x10³ dm³mol⁻¹cm⁻¹× Ctry × 1cm + 1.50x10³ dm³mol⁻¹cm⁻¹× Ctyr × 1cm <em>(2)</em>

<em></em>

Thus:

-2.7× (0.660 = 2.00x10³ × Ctry  + 1.12x10⁴ × Ctyr) =

-1.782 = -5.40x10³Ctry - 3.024x10⁴Ctyr

0.221 = 5.40x10³ × Ctry + 1.50x10³  × Ctyr

-------------------------------------------------------------------

-1.561 = -28740×Ctyr

<em>5.43x10⁻⁵ dm³mol⁻¹= Ctyr</em>

Replacing this value in (1) or (2) it is possible to find Ctry, that is:

<em>2.58x10⁻⁵ dm³mol⁻¹= Ctry</em>

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Jaką maksymalną ilość gramów azotanu (V) potasu można rozpuscic w 300g wody w temperaturze 90 C
olga2289 [7]

Answer:

  • <u>609g </u>

<u></u>

Explanation:

The translated question is:

<em>What maximum amount of grams of potassium nitrate (V) can be dissolved in 300g of water at 90 °C</em>

<em></em>

<h2>Solution</h2>

<em></em>

To answer the question you need to consultate the solubiity information for potassium nitrate (V), KNO₃.

The attached table contains the solutibility table for KNO₃ at different temperatures.

At 90ºC it is 203g / 100g water.

Then, to calculate the <em>maximum amount of grams of potassium nitrate (V) that can be dissolved in 300g of water at 90 °C</em>, just multiply by the amount of water:

  • 203g / 100g water × 300 g water = 609g ← answer

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3 years ago
Fter a radioactive atom decays, it is the same element that it was before with no measurable change in mass. Which kind of decay
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After a radioactive atom decays, it is the same element that it was before with no measurable change in mass. the decay that is present is gamma decay because gamma decay has photons which has no mass unlike alpha and beta decay.

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Answer:

Sorry I would bu that looks so hard

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What types of atoms typically form covalent bonds?

The correct answer:

d) Nonmetals with nonmetals, because their difference in electronegativity is below 1.7.

En example to explain:
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EN(Cl) = 3.0 and EN (H) = 2.1 (you can search these values in a periodic table)
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3 years ago
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Isopropyl alcohol is mixed with water to produce a 35.0 % (v/v) alcohol solution. How many milliliters of each component are pre
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229.25 mL of isopropyl alcohol

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Explanation:

Knowing that the solution have a 35.0 % (v/v) isopropyl alcohol in water, we can calculate the volume of each component as following.

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