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3 years ago

What is the role of NaCl and EDTA in DNA isolation?

1 answer:

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The following is the role of NaCl and EDTA in DNA isolation, hope it helps:

NaCl provides Na+ ions that will block negative charge from phosphates on DNA. Negatively charged phosphates on DNA cause molecules to repel each other. The Na+ ions will form an ionic bond with the negatively charged phosphates on the DNA, neutralizing the negative charges and allowing the DNA molecules to come together.

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5. Before vehicle emissions were well-regulated CO emissions were 66 g/mile. Assume this emission rate applies for an airshed. T

Aleks [24]

Answer:CALINE4 Model and Geographical Information System were used for

the present study to predict the CO concentrations and prepare thematic

maps for the study area.

CALINE4 is a latest model that predicts the concentration of carbon

monoxide impacts near the roadways. The California Department of

Transformation (CALTRANS) developed the model and its purpose is to help

planners protect public health from the adverse effects of carbon monoxide.

The model predictions along with the aid of GIS based model help to arrive at

air shed levels of carbon monoxide. CALINE4 is a simple line source

Gaussian plume dispersion model. The user defines the proposed roadway

geometry, worst-case meteorological parameters, anticipated traffic volumes

and receptor positions to predict the concentrations of pollutant.

CALINE4 is graphical with windows based user interface, designed to

ease data entry and increase the online help capabilities. The model was

developed for predicting the concentrations of relatively inert pollutants such

as carbon monoxide and it is now used for several other pollutants like NO2

and SPM. The model is based on fine tuned Gaussian diffusion equation and

it employs mixing zone concept to characterize the pollutant dispersion over

the roadways. Given the exhaust emission concentrations, meteorology and

site geometry, the model can predict the concentration of pollutants for any experimentation

Explanation:

4 0

3 years ago

Someone help me Explain how non-genetic factors can affect phenotype. Give an example.

Yanka [14]

Answer:

one could be the weather.

phenotypes are physical characteristics of a person and there non genetic factors that play a role.

weather and environmental factors, your surroundings and orders related to the environment basically

6 0

3 years ago

40 g of CaCO3 is how many moles of CaCO3? 10 moles 0.4 moles 40 moles 100 moles

AleksandrR [38]

Answer:

0.4 moles

Explanation:

To convert between moles and grams you need the molar mass of the compound. The molar mass of of CaCO3 is 100.09g/mol. You use that as the unit converter.

40gCaCO3* 1mol CaCO3/100.09gCaCO3 = 0.399640 mol CaCO3

This rounds to 0.4 moles CaCO3

8 0

3 years ago

please hurry! If 3.87g of powdered aluminum oxide is placed in a container containing 5.67g of water, what is the limiting react

VikaD [51]

Answer:

Explanation:

Given parameters:

Mass of aluminium oxide = 3.87g

Mass of water = 5.67g

Unknown:

Limiting reactant = ?

Solution:

The limiting reactant is the reactant in short supply in a chemical reaction. We need to first write the chemical equation and convert the masses given to the number of moles.

Using the number of moles, we can ascertain the limiting reactants;

Al₂O₃ + 3H₂O → 2Al(OH)₃

Number of moles;

Number of moles = $\frac{mass}{molar mass}$

molar mass of Al₂O₃ = (2x27) + 3(16) = 102g/mole

number of moles = $\frac{3.87}{102}$ = 0.04mole

molar mass of H₂O = 2(1) + 16 = 18g/mole

number of moles = $\frac{5.67}{18}$ = 0.32mole

From the reaction equation;

1 mole of Al₂O₃ reacted with 3 moles of H₂O

0.04 mole of Al₂O₃ will react with 3 x 0.04 mole = 0.12 mole of H₂O

But we were given 0.32 mole of H₂O and this is in excess of amount required.

This shows that Al₂O₃ is the limiting reactant

6 0

3 years ago

The pressure in a cylinder of nitrogen continuously decreases as a gas is released from it. On the other hand, a cylinder of pro

Juli2301 [7.4K]

Answer:

Explanation:

Nitrogen gas in nitrogen cylinder is in gaseous form only . So when gas is released , pressure decreases because moles of gas in the cylinder decreases .

Propane gas in cylinder is in liquified form . The gas is partially in gaseous and liquid form . So when gas is released outside , pressure decreases inside but due to low pressure , some liquid form is converted into gaseous form which restores the pressure of gas inside cylinder . Hence , the pressure of gas remains unchanged inside cylinder.

4 0

3 years ago