Penicillins disrupts bacterial cell wall synthesis.
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How does penicillin affect bacterial cell walls?</h3>
- Penicillin kills bacteria by inhibiting the proteins which cross-link peptidoglycans in the cell wall .
- When a bacterium divides in the presence of penicillin, it cannot fill in the “holes” left in its cell wall.
- β-Lactam antibiotics, including penicillins, cephalosporins, monobactams, and carbapenems, are distinguished by a lactam ring in their molecular structure and act by inhibiting the synthesis of the peptidoglycan layer of bacterial cell walls.
- Penicillins work by bursting the cell wall of bacteria. Drugs in the penicillin class work by indirectly bursting bacterial cell walls.
- They do this by acting directly on peptidoglycans, which play an essential structural role in bacterial cells.
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Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
It is needed by all living things and every living cell to carry out life processes, such as breaking down and building up molecules, and transporting many molecules across cell membranes. The form of energy that living things need for these processes is chemical energy, and it comes from food.
Answer:
B. Puerperium, or fourth trimester of pregnancy
Explanation:
- Puerperium or the fourth trimester of pregnancy is also known as the postpartum period.
- This period starts immediately after the mother gives birth to a child.
- During this period the size of the uterus and other reproductive organs along with the hormone levels return to their normal state.
- It is the phase where regression of all the anatomical and physiological changes that took place i in the reproductive organs of the females takes place.
- This phase is divided int three periods -
1. Immediate puerperium, or the first 24 hours after parturition
2. Early puerperium, which extends until the first week postpartum;
3. Remote puerperium, which includes the period required for involution of the genital organs and return of menses, usually approximately 6 weeks.
- This phase is highly critical for the mother as this requires rest and proper care as there are risks of bleeding. Therefore, the midwife or the nurse must take proper care of the mother.
