E) is the correct answer hope this helps :)
Answer:
C. Luria Agar + kanamycin + tetracycline
Explanation:
Given that the recipient strain is tetracycline resistance and the donor strain is tetracycline sensitive.
Recipient strain is kanamycin sensitive and the donor strain is kanamycin resistance sensitive.
If we grow the recipient cell in Luria agar containing tetracycline then only recipient cells will grow and all the donor cells will die.
If we grow the recipient cell in Luria agar containing kanamycin then only recipient cells that have undergone transposon mutagenesis will grow and along with donor cells.
If the Luria agar has both kanamycin and tetracycline then only the recipient cells that have undergone transposon mutagenesis will grow.
Since recipient cells will acquire pir gene. So it will not require pir in the media.
So correct answer is option C. Luria Agar + kanamycin + tetracycline
Internal fertilization has the advantage of protecting the fertilized egg from dehydration on land. The embryo is isolated within the female, which limits predation on the young. Internal fertilization also enhances the fertilization of eggs by a specific male.
Answer:
Legislation in some areas has been effective in preventing further coral reef loss. In Australia, zoning laws require individuals who have collector's licenses to collect only in certain areas. Collectors have been able to gather 45 to 50 metric tons of material with no measurable effect on coral reefs. Facilities have been established to measure cyanide levels in live reef fish. The percentage of fish testing positive for cyanide has declined from 80% to 30% in the past five years. Facilities have been able to make a positive impact on coral reef preservation efforts.
Explanation: