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2 years ago

What is restriction enzymes and what is it’s purpose?

Chemistry

1 answer:

Eddi Din [679]2 years ago

7 0

Answer: Endonuclease enzymes used in molecular biology that cut DNA at specified points.

Explanation:

Enzymes are specific protein types which bind to a substrate within a reaction, to increase the rate of reaction within the solution- they speed up the rate of reaction.

Restriction enzymes are bacteria-derived enzymes; these make cuts on deoxyribonucleic acid molecules or DNA. These are also called restriction endonucleases. They are utilized in molecular biology for DNA cloning and sequencing and cut DNA into smaller pieces called fragments.

Restriction enzymes make directed cuts on DNA molecules. They precisely target sites on DNA to produce mostly identical or homogenous, discrete fragments of equal sizes, producing blunt or sticky ends. In order to do this, they recognize sequences of nucleotides that correspond with a complementary sequence on the endonuclease called restriction sites.

There are several kinds that may require cofactors (chemical or metallic compounds that aid in enzyme activity) :

Type I: cleave far away from the recognition site; require ATP and SAMe S-Adenosyl-L-Methionine

Type II: cleave near to the site; require Magnesium

Type III: cleave near to the site; require ATP which is not hydrolysed but SAMe S-Adenosyl-L-Methionine is optional

Type IV: cleavage targeted to DNA that have undergone post transcriptional modification through certain types of methylation (addition of a methyl group)

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For the reaction shown, calculate how many grams of each product form when the following amounts of reactant completely react to

stira [4]

This may help you
You need to use some stoichiometry here. The only way to do that is if you're working in moles. Since you're given grams of Al, you can convert that moles by dividing by the molar mass. Then from looking at the coefficients in your equation, you can see that for however many moles of Al react, the same numbers of moles of Fe will be produced, but only half as many moles of Al2O3 will be produced. To go back to grams, multiply the moles of each product that you get by their molar masses!

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3 years ago

Which cup has more thermal energy?

dybincka [34]

Question: Which cup has more thermal energy?

A. 2 liter cup of milk

B. 1 liter cup of milk

Answer: A. 2 liter cup of milk

Explanation:

We have milk in both of the containers. The milk in both containers is the same temperature, so we will have to measure the amount of molecules. The amount of molecules makes it warmer, and if it is warmer, that is more energy. 2 liters have more thermal energy than 1 liter, because it has more molecules.

Our answer is 2 liter cup of milk.

5 0

2 years ago

Nitrifiers are bacteria

Delicious77 [7]

Answer:

(D.) Nitrifiers are bacteria that generate nitrites or nitrates.

Explanation:

In the nitrogen cycle which occurs in nature, ammonia and ammonium compounds in the soil from organic sources and are converted to nitrites and nitrates by aerobic microorganisms.

Nitrifiers, as the name implies, are these such aerobic bacteria which oxidize inorganic constituents in the soil to generate energy. Examples of these nitrifiers are nitrobacter and nitrosomonas.

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2 years ago

Determine the LIMITING reactant in the following balanced equation:

padilas [110]

Answer:

KBr is limiting reactant.

Explanation:

Given data:

Mass of KBr =4g

Mass of Cl₂ = 6 g

Limiting reactant = ?

Solution:

Chemical equation:

2KBr + Cl₂ → 2KCl + Br₂

Number of moles of KBr:

Number of moles = mass/molar mass

Number of moles = 4 g/ 119 gmol

Number of moles = 0.03 mol

Number of moles of Cl₂:

Number of moles = mass/molar mass

Number of moles = 6 g/ 70 gmol

Number of moles = 0.09 mol

Now we will compare the moles of reactant with product.

KBr : KCl

2 : 2

0.03 : 0.03

KBr : Br₂

2 : 1

0.03 : 1/2×0.03= 0.015

Cl₂ : KCl

1 : 2

0.09 : 2/1×0.09 = 0.18

Cl₂ : Br₂

1 : 1

0.09 : 0.09

Less number of moles of product are formed by the KBr thus it will act as limiting reactant while Cl₂ is present in excess.

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2 years ago

How do cells respond to contact with other cells?

Blababa [14]

It depends on the function and activity of both cells. Most normal cells crease division if they came into contact with other cells however the immune system interact directly with pathogens or foreign organisms to destroy them.

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3 years ago