1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Vaselesa [24]
3 years ago
7

Place the primers in the correct orientation and locations to amply this gene by pcr. if a primer does not belong in a particula

r location, place the label with an "x" in that location. note that the arrows on the primers represent the direction of dna synthesis.
Biology
2 answers:
Alika [10]3 years ago
7 0

Primers should be located in the 5' end of the sequence because only in this direction, DNA polymerase enzyme work properly.  

Further Explanation:

DNA synthesis is a very organized and highly systemic process. The addition of bases in both strands takes place from 5’ to 3’ end of the new DNA strand using all the DNA polymerase. The only difference is the continuous and discontinuous synthesis of DNA in leading and lagging strands, respectively. Three types of DNA polymerase are found, and each has some specific function during DNA replication. A common feature of all three polymerases is the addition of nucleotides from 5’ to 3’ direction of the new DNA strand.

DNA primase plays an essential role in the synthesis of primers. These primers, in turn, are used by DNA polymerase to synthesize new strands. DNA polymerase requires primers to start the synthesis of the new strands. Enzyme primase is responsible for the synthesis of these primers. In vitro DNA synthesis occurs with the help of PCR. To amplify a genetic sequence with the help of polymerase chain reaction require a reverse primer and forward primer.

Learn More:

1. Learn more about the treatment of eukaryotic cell with a drug <u>brainly.com/question/10767798 </u>

2. Learn more about the proteins synthesis in a cell <u>brainly.com/question/1420458 </u>

3. Learn more about the exchange of gases by blood cells <u>brainly.com/question/1213217 </u>

Answer Details:

Grade: High School

Subjects: Biology

Chapter: Molecular Biology

Keywords:

DNA polymerase, synthesis, primer, PCR, forward, primer, continuous, strand, systemic feature, leading lagging, replication, primase.

galina1969 [7]3 years ago
4 0

DNA replication is the process of doubling a DNA double chain. In cells, DNA replication occurs before cell division. Prokaryotes continually replicate DNA. In eukaryotes, the timing of DNA replication is highly regulated, ie in the S phase of the cell cycle, before mitosis or meiosis I. The multiplication utilizes the DNA polymerase enzyme which helps form bonds between the nucleotides that make up the DNA polymer. The process of DNA replication can also be carried out in vitro in a process called a polymerase chain reaction (PCR).

<h2>Further Explanation </h2>

A slow strand (Lagging strand) is a DNA strand located on the opposite side of the leading strand on the replication fork. These strands are synthesized in segments called Okazaki fragments. In this string, primases form RNA primers. The DNA polymerase can thus use OH 3 'free groups in the RNA primer to synthesize DNA in the direction of 5' → 3 '. The primary RNA fragments are then removed (for example by RNase H and DNA Polymerase I) and new deoxyribonucleotides are added to fill the gaps that were previously occupied by RNA. DNA ligase then connects the Okazaki fragments so that the synthesis of lagging strands is complete.

Primers both on the steering strand and on the lagging strand will elongate with the help of Holoenzyme DNA polymerase III. This multisubunit complex is a dimer, half will work on the steering strand and the other half will work on lagging strands. Thus, the synthesis of the two strands will run at the same speed.

Each dimer part of the two strands consists of subunit a, which has the actual polymerase function, and subunit e, which has an editing function in the form of exonuclease 3'– 5 ’. In addition, there is a subunit b that attaches polymerase to DNA.

Once the primers in the remaining strand are removed by DNA polymerase III, they will be removed immediately and the gaps caused by the loss of the primer are filled with DNA polymerase I, which has 5 '- 3' polymerase activity, 5 '- 3' exonuclease, and editing 3 exonuclease '- 5'. Eksonuklease 5 '- 3' discard the primer, while the polymerase will fill the gap caused. Finally, the Okazaki fragments will be united by the DNA ligase enzyme. In vivo, the dimoenzyme DNA polymerase III and primosomes are believed to form large complexes called replisomes. With the replisom DNA synthesis will take place at 900 bp per second.

Learn more

DNA replication brainly.com/question/5932348

Details

Grade:  College

Subject:  Biology

keywords: DNA, RNA, replication.

You might be interested in
a race car driver drives one lap around a track that is 500 meters in length. what is the drivers displacement at the end of the
sleet_krkn [62]

Answer:

0 meters

Explanation:

When we talk about displacement, think that the think of it as the measure of the distance between the starting point of the object, from its end point.

Since we are talking about a lap around a track, it would mean that the starting point is also the end point. So if you started and end at the same point, the distance between it would be 0.

5 0
3 years ago
If plants are absorbing and using blue and red light, what must be happening to other colours of light, like green and yellow?
Semenov [28]

Answer:

The colours like green and yellow are being reflected.

As we know if the colour is green then it is reflecting green light

Explanation:

8 0
2 years ago
Read 2 more answers
Why are the ventricles of the heart so much larger than the atria of the heart?
konstantin123 [22]
<span>The ventricles are the part of the heart that are responsible for the pumping action to move blood throughout the body. Therefore, the muscles in the ventricles are much larger and thicker to do their job properly. The atrium only has to pump blood through an open valve (the tricuspid valve) into the right ventricle. There is much less resistance to this flow of blood so much less muscle is needed. </span>
7 0
3 years ago
A pharmaceutical company claims that it has developed a drug that will decrease the likelihood of seizures in patients with epil
Paraphin [41]

Answer:

B

Explanation:

This ensures that the research is corroborated by other scientists and is peer-reviewed hence increasing the veracity of the findings. This also ensures that conflict of interest is addressed whereby the findings by the company may appear to be compromised or biased because such a finding would be in favour of their own needs (such as financial gain).  Peer review in such case is  even more important because it involves humans subjects and ethics are paramount in healthcare research.

6 0
3 years ago
Read 2 more answers
Round pea allele R is dominant to wrinkled pea allele r, while yellow pea allele Y is dominant to green pea allele y. Homozygous
allsm [11]

Answer:

RrYy (F1 generation)

Explanation:

This is a typical dihybrid cross involving two genes in the pea plant; one coding for seed colour and the other for seed shape.

According to Mendel's law of dominance, an allele is capable of masking another allele in a gene, the allele that masks or is expressed is called the DOMINANT allele while the allele being masked is called RECESSIVE allele.

Homozygosity of a gene refers to it having the same type of alleles while heterozygosity refers to having different alleles.

In this example, allele for Round (R) and Yellow (Y) pea is dominant over the allele for Wrinkled (r) and green pea respectively.

Homozygous round green pea (RRyy) crossed with Homozygous wrinkled yellow (rrYY) will give rise to a heterozygous round yellow (RrYy) F1 offsprings.

4 0
3 years ago
Other questions:
  • Maria's favorite candy bar has 230 calories. the nutrition label states that 7/8 of the calories come from fat.how many calories
    15·1 answer
  • Which characteristic is more indicative of bulimia than anorexia nervosa?
    8·1 answer
  • What is the common name for the group that snails slugs clams and squids
    14·1 answer
  • A student designed an experiment to determine if air temperature had an effect on the rate of photosynthesis in corn plants. A c
    7·1 answer
  • Why is biochemical evidence the strongest form of evolutionary evidence?
    13·1 answer
  • This polysaccharide is the most abundant organic compound on
    8·1 answer
  • What is the relationship between air pressure and winds
    13·2 answers
  • Which of the following types of rock would most likely contain a fossil?
    9·2 answers
  • What is a peroxisome?
    14·2 answers
  • Spermiogenesis begins with a ________ and ends with a ________.
    9·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!