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Delicious77 [7]
3 years ago
12

Peptides can be separated using an ion‑exchange column based on their isoelectric (pI) values. At which pH values would two diff

erent peptides, one with a pI of 5.5 and the other with a pI of 9.1 , bind to a cation‑ and anion‑exchange column? Each peptide may be capable of binding to each column at more than one pH value.
Chemistry
1 answer:
Angelina_Jolie [31]3 years ago
7 0

Answer:

The ansers are in the explanation.

Explanation:

The isoelectric point of a peptide is the pH in which this compound will be electrically neutral. When the pH is lower than pI, the peptide will be a cation. when the pH is higher than pI, the peptide will be as an anion.

Cation‑ and anion‑exchange columns are chromatography columns that allows the separation of substances based in their charges. A cation-exchange column will bind to positively charges molecules and an anion-exchange column will bind with negatively charges molecules.

Thus, the peptide with pI of 5,5 will bind with cation-exchange column at pH's lowers than 5,5 and will bind with anion-exchange column at pH's highers than 5,5.

In the same way, the peptide with pI of 9,1 will bind with cation-exchange column at pH's lowers than 9,1 and will bind with anion-exchange column at pH's highers than 9,1.

I hope it helps!

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A solution has an absorbance of 0.2 with a path length of 1 cm. Given the molar absorptivity coefficient is 59 cm⁻¹ M⁻¹, the molarity is 0.003 M.

<h3>What does Beer-Lambert law state?</h3>

The Beer-Lambert law states that for a given material sample, path length and concentration of the sample are directly proportional to the absorbance of the light.

A solution has an absorbance of 0.2 with a path length of 1 cm. Given the molar absorptivity coefficient is 59 cm⁻¹ M⁻¹, we can calculate the molarity of the solution using the following expression.

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Learn more about the Beer-Lambert law here: brainly.com/question/12975133

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