Mitosis is a part of the cell cycle when replicated chromonsomes are separated into two new nuclei.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
No
Explanation:
The Sun is a star there are lots of stars,but the Sun is the closest one to Earth.It made from gases and it always burning
Planets are:Mercury, Venus, Earth, Mars, Jupiter, Saturn, and Uranus
This is because direct power has no pulsation (waveform characteristic) that is significant in electromagnetic induction. The gradual increase and decrease of power, at a given frequency, in indirect power is significant in electromagnetic induction. This enables transformers to induce electricity into the secondary coil, from the primary coil, at the core of the transformer.Transformers transform electric power by increasing or decreasing the voltage and current of electricity.
A) Crossing over is the mechanism of genetic variation that results from the exchange of gene segments between non-sister chomatids.
