Answer;
-HaeIII cuts at the sequence GG:CC which is found at nucleotide 143-146 of the TAS2R38 gene. A non-taster alley changes the sequence at position 145 from a C to a G. This makes the gene no longer recognized by the restriction enzyme.
Explanation;
-Restriction enzymes recognize and make a cut within specific palindromic sequences, known as restriction sites, in the genetic code. This is usually a 4- or 6 base pair sequence.
For example; HaeIII is a restriction enzyme that searches the DNA molecule until it finds this sequence of four nitrogen bases.
5’ TGACGGGTTCGAGGCCAG 3’
3’ ACTGCCCAAGGTCCGGTC 5’
Once the recognition site was found HaeIII could go to work cutting (cleaving) the DNA
Random sampling means that members of a 'population' have equal chances of being selected. To carry out this type of sampling, you will need to use a table of random numbers.
It enters the heart via the pulmonary veins