When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:
The correct answer is: semi-conservative.
Explanation:
DNA replication is an important process in which the DNA is duplicated before cell division so each daughter cell can have a copy of genetic material.
DNA replication is <u>semi-conservative because one strand of DNA is new and the other one is the old strand</u>. To replicate itself, the DNA double helix is opened, and then each strand serves as a model for the new strands.
DNA replication is <u>not a one-step process</u>, as it takes several complicated steps in which <u>lots on enzymes participate</u> (like DNA polymerase and DNA helicase).
DNA replication is <u>also carried by prokaryotic cells</u>, though it's a much simpler process.
Answer:
As we know 10% law is transfer of energy from higher trophic level to lower trophic level. So as the energy are transfer from one organism to another there is a loss of energy as that energy are utilised by the oragnism and only 10% of the total energy is transferred to the second consumer.
Explanation:
Quetinary consumer are at the higher position in the food chain so they do not get enough energy from prey they have to feed on several organism for the enegy. And also they are at the top of the food chain so they are not eaten by other animal, their life span are also more than primary consumer.
In other hand primary consumer get enough energy but they are prey for other higher tropic level.
Primary and secondary consumer are maintend in such a way by the nature thats make a ecological balance of the habitant.
Answer:
These homologous chromosomes represent a maternal and a paternal chromosome
Explanation:
