Why does using two different enzymes to cut the plasmid prevent the plasmid from reforming a circle without the inserted gene?

1 answer:

7 0

Because they do not have compatible sticky ends, <span>to treat both the gene of interest and our plasmid vector with the same restriction enzymes because they would have compatible sticky ends. </span><span>The insert has a chance of combining with the plasmid. To ensure efficient digestion, the two recognition sites should be more than 10 base pairs apart. </span>

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