Answer:
(2) Half of the active sites are occupied by substrate.
Explanation:
The Michaelis–Menten equation is the rate equation for a one-substrate enzyme-catalyzed reaction. It is an expression of the relationship between the initial velocity V₀ of an enzymatic reaction, the maximum velocity Vmax, and substrate concentration [S] which are all related through the Michaelis constant, Km.
Mathematically, the Michaelis–Menten equation is given as:
V₀ = Vmax[S]/Km + [S]
A special relationship exists between the Michaelis constant and substrate concentration when the enzyme is operating at half its maximum velocity, i.e. at V₀ = Vmax/2
substituting, Vmax/2 = V₀ in the Michaelis–Menten equation
Vmax/2 = Vmax[S]/Km + [S]
dividing through with Vmax
1/2 = [S]/Km + [S]
2[S] = Km + [S]
2[S] - [S] = Km
[S] = Km
Therefore, when the enzyme is operating at half its maximum velocity, i.e. when half of the active sites are occupied by substrate, [S] = Km
First, we need to get moles of HA =molarity * volume
= 0.1 m * 0.075 L = 0.0075 moles
moles of NaOH = molarity * volume
= 0.1 * 0.03 L = 0.003 moles
from the reaction equation:
HA(aq) + NaOH(aq) → NaA(aq) + H2O(l)
that means the final moles' HA = 0.0075 - 0.003 =0.0045 moles
when the total volume is = 0.075 + 0.03 L = 0.105 L
∴ [HA] = moles / volume
= 0.0045 / 0.105 L = 0.043 m
[A^-] = 0.003 / 0.105 L = 0.029 m
then by using H-H equation:
PH = Pka + ㏒[A^-] / [HA]
by substitution, we can get Pka:
5.5 = Pka+ ㏒ (0.029 /0.043)
∴ Pka = 5.67
when Pka = - ㏒Ka
5.67 = -㏒ Ka
∴Ka = 2 x 10^-6
Answer:
Matter is the Stuff Around You
Matter is everything around you. Atoms and compounds are all made of very small parts of matter. ... Matter is defined as anything that has mass and takes up space (it has volume).
Explanation:
hope it was helpful
Answer= storage
Good luck