Answer:
5.88 μM is the concentration of red dye in the sample.
Explanation:
Absorbance is defined s capacity of a substance to absorb a light of a specified wavelength.It is calculated by using Beer-Lambert Law:
A = absorbance
= Molar absorptivity
c = concentration
l = Length of the solution through which light passes
Given, A = 0.468, l = d = 1.00 cm,c = ?
(
)
5.88 μM is the concentration of red dye in the sample.
The mass is 132.0384 grams.
Answer:
The Retention factor (rf) value is = 0.2
Explanation:
- Retention factor (Rf) is factor used substances that could be separated using Chromatography. Retention factor determines how fast the component can move on the chromatogram (stationary phase) after elution. Elution occurs when mobile phase (solvent) moves across the stationary phase when the solute has been spotted on the origin.
- Retention factor (Rf) ranges from value between 0 and 1. The closer the value to 1, the faster it can move upon elution. Rf can be calculated.
- Rf value = distance moved by the solute / distance moved by the solvent
= 0.40cm / 2.00cm
= 0.2
