Definition
A technique which is used to separate, DNA, RNA or protein pieces from each other under the influence of electric field on the basis of their molecular size is known as gel electrophoresis.
Explanation
This method is very reliable for separation of large size molecule (over 1 million Da). Materials which are required for gel electrophoresis include:
1. TAE stock buffer
2. 1% agrose gel
3. Nucleic acid loading dye
4. Ethidium bromide
Procedure:
First prepare a stock solution of TAE buffer by adding appropriate amount of TAE in distilled water. then prepare 1% agrose by adding 1X TAE, some amount of agrose in water and heating it in microwve oven to mix them will. then pour agrose gel on tray and fix comb in it and keep it untill agrose dry. Then remove comb and pour some quantity of nucleic acid along with loading dye and ethidium bromide in each well. EtBr is used for staining nucleic acid.
When sample is poured in all well also pour reference marker in one well for comparison. now connect it with voltage for 30-35 min. After this take gel and see it under UV. a large number of nucleic acid pieces will be seen on gel under UV. those pieces which have small molecular weight will cover more distance compared to those having larger molecular weight.
Japanese beetles store the lipids they need in proteins and during mating, to produce offspring they combine the male's proteins with the female's protein.
Hope it helped,
BioTeacher101
Answer:
The test statistic for this hypothesis test is - 3.68.
Explanation:
A test statistic is a random variable that is calculated from sample data and used in a hypothesis test. You can use test statistics to determine whether to reject the null hypothesis. The test statistic compares your data with what is expected under the null hypothesis.
Sample proportion = 38/50
= 0.76
Hence,
Test statistic
= 3.68
Answer:
?? I don't know what these means
Answer:
When the frequency of stimulation of a muscle increases -without it having the opportunity to relax- a process called summation (addition) occurs, promoting an increase in the generation of force in the isolated skeletal muscle.
Explanation:
Summation is a phenomenon that occurs as a consequence of the arrival of successive stimuli that produce the contraction of the skeletal muscle before it achieves its partial or total relaxation, between subsequent stimuli.
<em>When the summation occurs in the muscle, the force generated on it increases its magnitude proportionally to the number of stimuli received, maintaining the muscle contraction in time</em>.
Tetany is the prolonged contraction of a muscle in an abnormal way, by the summation of stimuli received , as some bacterial toxins can produce. The summation can be temporary -when multiple stimuli reach the muscle in a determined time- or spatial, when the amount of stimuli activates a greater amount of motor units.
Learn more:
Spatial summation in a post synaptic neuron brainly.com/question/9632682
