Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer:
i think it is all atoms of all elements are exactly alike and have the same mass
Explanation:
If the O-Zone Tropo increased to a point, like it has, it can cause a huge variety of health problems and even death! Some of the problems include, but are not limited to: Asthma, E<span>mphysema, Chest Pain, Inflamed Lungs and Lung Scarring from tissue damage. </span>
Answer:
Vapour pressure of benzene over the solution is 253 torr
Explanation:
According to Raoult's law for a mixture of two liquid component A and B-
vapour pressure of a component (A) in solution = 
vapour pressure of a component (B) in solution = 
Where 
are mole fraction of component A and B in solution respectively
are vapour pressure of pure A and pure B respectively
Here mole fraction of benzene in solution is 0.340 and vapour pressure of pure benzene is 745 torr
So, vapour pressure of benzene in solution = 
= 253 torr
We can't use the nitrogen present in the atmosphere although 80% of the atmosphere consists of nitrogen only.We get this from the plants called as leguminous plants which can convert or fix atmospheric nitrogen into usable ones..
