Well for the latitude add 5 but for the longitudei dont know, try adding 2 and find the pattern.
Your answer should be tissues.
If a linear piece of DNA has three sites for a particular restriction enzyme, it will be cut into 4 fragments. If the DNA is circular, the number of obtained fragments will be N for N recognition sequences for the specified restriction enzymes however if the DNA is linear, the number of obtained fragments will be (N+1).
A bacterial protein called the restriction enzyme cleaves DNA at specific locations. The recognition sequences, or short and distinct nucleotide sequences, are recognized by restriction enzymes in DNA. When a DNA sequence is recognized by the restriction enzyme, it hydrolyzes the bond between neighboring nucleotides and cleaves the DNA molecule.
The restriction enzymes guard against bacteriophages on the living bacteria. They identify the bacteriophage and cleave at its restriction sites, destroying its DNA.
The use of restriction enzymes in genetic engineering is crucial. In order to introduce foreign DNA for gene-editing purposes, restriction enzymes cut DNA at a specified location, which makes them a crucial tool.
To learn more about restriction enzyme, recognition sequences and bacteriophages here
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I believe the first answer is #4 and the second is #1. Please correct me if I am wrong.
Answer and explanation
1. Cloning differs from budding as it generates a genetically identical COPY of a cell or organism while budding produces a genetically identical offspring.
2. Firstly it can take place quickly, allowing the pioneer species to establish themselves in their new habitat, out competing slowly -reproducing species.
Secondly since all the offspring are genetically alike, it ensures that if the parents are successful in their habitat, the offspring will also be successful, provided environmental conditions do not change .
3. Plant cloning uses one of the plant organs can be roots , stem and etc ..while animal cloning only uses a single cell