Question

Absorbance measurements in the range of A = 0.3-2 are considered the most accurate. Why would absorbance measurements of 0.05 and 2.5 be considered inaccurate? Select all that apply.1. At A = 2.5, the detector is saturated because too much light is reaching the detector.2. The difference in the absorbance of the sample and reference is too small at A = 0.05.3. If the absorbance is too high (A = 2.5), the signal difference between the sample and the reference is too small for an accurate measurement.4. At A = 2.5, a sufficient amount of light does not reach the detector for an accurate measurement.5. At A = 2.5, the transmission is too high for an accurate measurement.

Answer 1

Answer:

The difference in the absorbance of the sample and reference is too small at A = 0.05.3. If the absorbance is too high (A = 2.5),