C. spinal nerves
it is the answer to your question.
Answer:
i believe it's sharp and strong.
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Answer:
A) The resources used by both species of salamanders are available in limited supply
B) Body size is a heritable trait in salamanders Salamanders that are closer in body sine compete less strongly for food
C) The sympatric salamander populations evolved their present body sizes afterthey became sympatric
D) In sympatric populations, smaller P. Cinereus adults survive better and reproduce more.
All of the above are correct.
Chinese hamster ovary cell production of recombinant tissue‐type plasminogen activator (t‐PA) was increased by amplification of cotransfected dihydrofolate reeducates cDNA using stepwise adaptation to increasing methotrexate (MTX) concentrations. The highest producing clones were isolated at 5 μM MTX and yielded 26,000 U/106 cells/day t‐PA (43 μg/106 cells/day). Above 25 μM MTX, cell specific t‐PA production rates became increasingly variable and the cDNA copynumbers decreased. No apparent correlation between the cell specific t‐PA production rate and the growth rate was observed upon sub cloning of the amplified cells. When MTX selection was removed, the t‐PA production rate decreased up to tenfold within 40 days; this was accompanied by an up to 60% drop in cDNA copynumber. Subclones isolated after 108 days of culture in the absence of MTX were, on average, sixfold more stable than their parental cells. In culture without MTX, the maximum stable t‐PA production rate obtained (over 250 days) was 7000 ± 750 U/106cells/day (∼12 μg/106 cells/day), approximately threefold lower than the maximum unstable levels of production reached under selective pressure. Taken together, these results define a wide range of the highest t‐PA expression rates obtained under MTX selection, for which stable expression without selection has not been reported
Answer:
hope this is right.
Brine rejection is a process that occurs when salty water freezes. The salts do not fit in crystal structure of water ice, so that salt expelled. Since the oceans are, this process is important in nature. In better understanding, as salt water freezes, the salt is pushed out of solution through channels in the ice. This process is called brine rejection or brine exclusion.