Fungi lacks the ability to produce their own food because they do not have chloroplast which is needed to obtain energy from the sun and convert it into food. Also fungi lack ability to fix nitrogen which has to be incorporated into their food. They also can not fix carbon and utilize it to produce food. Because of these they have to depend on dead organic matters for food.
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Most of these techniques, including microarray analysis and reverse transcription polymerase chain reaction (RT-PCR), work by measuring mRNA levels. However, researchers can also analyze gene expression by directly measuring protein levels with a technique known as a Western blot.
A theory in science is a remark on what happened
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Well , I think the answer is B
This represents a closed system,and one benifit is that the risk of passing disease between other species is lower than in open system
But still I prefer you to check it out in your book