Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer: Entropy is sometimes described as the degree of randomness in a system.
Answer:N
2
+ 3
H
2
-----> 2N
H
3
Explanation:
N
2
+
H
2
-----> N
H
3
Let us balance this equation by counting the number of atoms on both sides of the arrow.
N
2
+
H
2
-----> N
H
3
N=2 , H=2 N=1, H=3
To balance the number of N atom on Right Hand Side (RHS) , I will add one molecule of N
H
3
on RHS
N
2
+
H
2
-----> 2N
H
3
N=2 , H=2 N=2 , H= 6
To balance the number of H atoms on Left Hand Side (LHS) , I will add two molecules of
H
2
on LHS
N
2
+ 3
H
2
-----> 2N
H
3
N=2 , H=6 N=2 , H= 6
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NaCl and H2S will experience dipole-dipole interaction because they are permanently polarized.
Answer : The molecular formula of the compound is, 
Explanation :
Molecular formula : It is the representation of substance by the symbols and it denotes the number of atoms of each element present in the compound.
Now count the number of carbon, hydrogen, nitrogen and oxygen atoms present in the given compound.
As we see that in the given compound, there are 8 atoms of carbon element, 3 atoms of oxygen element, 1 atom of nitrogen element, 9 atoms of hydrogen element.
Thus, the molecular formula of the compound will be
