Answer:
(a) 133.33nm
(b) 600nm
(c) 7,600nm
Explanation:
The concentration of Y can be determined by using the formula:
where;
[L] = concentration of the binding ligand.
kd = 400 nm
Thus:
When Y = 0.25; we get :
0.25 (400 + [L]) = [L]
100 + 0.25[L] = [L]
100 = [L] - 0.25 [L]
100 = 0.75 [L]
[L] = 100/0.75
[L] = 133.33 nm
At, Y = 0.6
0.6 (400 + [L]) = [L]
240 + 0.6[L] = [L]
240 = [L] - 0.6 [L]
240 = 0.4 [L]
[L] = 240/0.4
[L] = 600 nm
At, Y = 0.95
0.95 (400 + [L]) = [L]
380 + 0.95[L] = [L]
380 = [L] - 0.95 [L]
380 = 0.05 [L]
[L] = 380/0.05
[L] = 7600 nm