Question

Which of the following is true regarding cultivation and isolation of animal viruses? View Available Hint(s) Which of the following is true regarding cultivation and isolation of animal viruses? Mouse models are available for virtually all human viral infections and can be routinely used for vaccine development. The preferred and widely used method of viral isolation and growth is via growth in an embryonated egg. Viruses can be easily grown in liquid culture without any other organisms present. Diploid cell culture lines, developed from human embryos, are widely used for cult

Answer 1

completed question'

.....culturing viruses that requires a human host

Answer:

Diploid cell culture lines, developed from human embryos, are widely used for culturing viruses that require a human host

Explanation

Viruses can not thrive in a non-living host or artificial media.They  are intracelular parasites  which needed living host to replicate . Cultures lines from Human embryo  in are therefore used for culturing viruses of human host, so that its mode of replication and gene expression can be studied, and therefore its virulence can easily be studied.

This method have the advantage that;

1.there is no need to make use of the whole animal rather,on a tiny tissue needed can be isolated for culture.

2. the cells growth is continuous,and can be preserved in liquid Nitrogen and renew for future culture

3. cells can be grown in different containers,  with ability to decide the number of cells needed.

Temperature is kept at optimum for human in the culture at  37 degree centigrade, nutrients are provided, NaHC03 as buffers for C02, and  the medium is humidified.