Question

Suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture I it and told you to make a 10^(-1) dilution of the entire culture. Explain how you would do this. Show your calculations

Answer 1

Answer:

Please see below for the answer

Explanation:

A $10^{-1}$ dilution is the same with 1:10 dilution in which one part of the stock solution is mixed with nine part of solvent. In this case, the solvent being water.

In order to prepare $10^{-1}$ dilution from a 2.0 mL of an E. coli broth, the 2 mL broth will be pipetted into 18 mL of distilled water in a test tube.

Calculation

2 mL broth in 18 mL distilled water = 2/20 dilution.

2/20 = 1/10 dilution

1/10 = $10^{-1}$ dilution.