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Soloha48 [4]
4 years ago
10

Protein x has an absorptivity of 0.4 ml·mg-1 ·cm-1 at 280 nm. What is the absorbance at 280 nm of a 2.0 mg ·ml-1 solution of pro

tein x? (assume the light path is 1cm).
Chemistry
1 answer:
Evgen [1.6K]4 years ago
3 0

Absorbance measures the ability of the substance to absorb light at a specific wavelength.

Absorbance is also equal to the product of molar absorptivity, path length and molar concentration.

The mathematical expression is given as:

A= \epsilon l c       (1)

where, A = absorbance

\epsilon =  molar absorptivity

l = path length

c  = molar concentration.

The above formula is said to Beer's Law.

Absorptivity of protein x  = 0.4 mLmg^{-1}cm^{-1}

Path length = 1 cm

Molar concentration = 2.0 mg mL^{-1}

Put the values in formula (1)

Absorbance at 280 nm = 0.4 mL mg^{-1}cm^{-1}\times 1 cm \times 2.0 mg mL^{-1}

= 0.8

Thus, absorbance at 280 nm = 0.8

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