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ankoles [38]
3 years ago
6

Transformation is a useful technique for making genetically modified bacteria in the laboratory. There have been efforts to tran

sform Archaea, but it has been more difficult than transforming Bacteria. One reason that it has been difficult is that Archaea lack a peptidoglycan cell wall. Why does that affect the ability of researchers to develop techniques to transform them
Biology
1 answer:
Mama L [17]3 years ago
5 0
<h2>Transforming Bacteria</h2>

Explanation:

  • Archaea and bacteria are different in the qualities of the cell wall and in the lipid composition of their cell membranes
  • <em>Bacterial cell dividers contain peptidoglycan</em>
  • Archaean cell wall don't have peptidoglycan, yet they may have <em>, polysaccharides, glycoproteins, or protein-based cell dividers </em>
  • Bacterial cell walls are made of peptidoglycan (likewise called murein), which is produced using polysaccharide chains cross-connected by abnormal peptides containing D-amino acids
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2. Following her visit to the pediatrician, Cindy undergoes a diabetic care training program, learning how to self-inject insuli
garri49 [273]

Full question  below

Answer:

1. The elevation is due to failure of the beta-cells of the pancreas to produce hormone insulin, which  facilitates entry of   blood glucose into cells via GLUT  transporters. Therefore  the level of  blood glucose rises, beyond threshold.

2. Lower blood pH is  increase in blood acidity.  it is due to the process of Gluconeogenesis; (conversion of non -carbohydrate molecule to glucose) where fatty acid is withdrawn from  adipose tissues and broken down to  ketone bodies by beta oxidation in the liver.Ketonebodies is acidic, therefore lowers the blood pH.(metabolic acidosis) this is complication is called Diabeticketoacidosis(DKA) and it is very common in type 1 diabetes mellitus.

3. the fruity odour is a result of ketone  acidosis accumulation in the blood.  the smell is that of acetone. this is a volatile  ketone that can be released in the lungs. Due to  metabolic acidosis, the body compensatory mechanism, buffer up the hydrogen ions; by excretion in the kidney,  haemoglobin bonding with formation of <u>haemoglobinic</u> acid(HBB) and increases  in breathing -hyperventilation to  reduce blood C02 levels.

4.Frequent urination is due to elevated blood glucose levels from gluconeogenesis and glucogenlysis. The high glucose levels is excreted in the kidney, ans since the blood solute potential has increased,water flows out via osmosis. therefore large diluted urine is produced (polyuria); leading to dehydration.

5.The failure of  glucose uptake by the cells, and constant massive  loss in the urine leads to failure of  cellular metabolic reaction and  starving of the body cells.The satiety(hunger)center in the hypothalamus is stimulated and Cindy feels hunger pangs. However, in normal conditions GLUT transporters(membrane protein channels) under  the regulation  of insulin facilitates the uptake of glucose into the cells, for metabolic activities.These transporters are designated;e.g GLUT 1, (brain)GLUT 2,(liver) GLUT 4.(muscles)

1. she has type 1 diabetes mellitus,  because of her age and most of her complications which are  due to absence of  hormone insulin in the blood streams, especially her metabolic acidosis,<u>polyuria,</u> <u>dehydration,and hunger pangs.</u>

2 this can lead to hypoglycemia, low blood glucose level as  excessive insulin withdraws  blood glucose,into the cells through the  GLUT transporters. This depletes blood glucose leading to tiredness, confusion,fainting and constipation. It also leads to activation of <u>sodium-potassium ATPases</u> in the membrane cells, which increases the movements of excessive <u>potassium ions into the cells.</u> This leads to Hyperkalemia.

she needs to constantly maintain her blood glucose level to the threshold; because of massive losses in the kidney.<u>Therefore, she needs candy to supply direct glucose to the cells from the blood, and  to placate her thirst from polyuria and dehydration,by keeping  her mouth moist, </u>

and

<u>hormone glucagon to break down glycogen to glucose to  maintain blood glucose threshold level to the cells.</u>

Explanation:

Chief Complaint: 8-year-old girl with excessive thirst, frequent urination, and weight loss. History: Cindy Mallon, an 8-year-old girl in previously good health, has noticed that, in the past month, she is increasingly thirsty. She gets up several times a night to urinate, and finds herself gulping down glassfulls of water. At the dinner table, she seems to be eating twice as much as she used to, yet she has lost 5 pounds in the past month. In the past three days, she has become nauseated, vomiting on three occasions, prompting a visit to her pediatrician. Questions A. At the doctors office, blood and urine samples are taken. The following lat resuits are noted blood glucose level 545 mg/dl (normal 50 170 mg/di) blood pH level 7.23 urine-tested positive for glucose and for acetone acetoacetate (ie. ketone bodies) (normally urine is free of glucose and ketone bodies) (normal 7.35-7.45) 1. Why is her blood sugar level elevated? (2points) 2. Why is her blood pH level decreased? (2 points) 3. Cindy has a fruity odor to her breath. Why? (2 points) 4 Explain why Cindy is urinating so frequently? (2 points) 5. How is Cindys condition like that of starvation? Address the role of glucose transport in your answer. (3 points) B. Following her visit to the pediatrician, Cindy undergoes a diabetic care training program, learning how to self-inject insulin subcutaneously and check her blood- glucose level at home with chemstrips. In addition, she learns the importance of carrying candy and glucagon with her at all times as well as eating the right amounts of food at the right times each day 6. Given that she is being treated with insulin, what type of diabetes does she have? What dangers confront Cindy as she gives herself insulin artificially ona daily basis? Why must she carry candy and glucagon with her at all times? (3 points)

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