Answer:
a) [Tris0] : [Tris] = 1 : 100
b) Range = 7.1 to 9.1
Explanation:
a) Calculation of ratio of the basic and the acidic forms of tris
pH of a buffer is calculate using Henderson-Hasselbalch equation

Conjugate acid of Tris dissociated as

For tris,
Salt or Basic form = tris0
Acid or Acidic form = Tris
pKa = 8.1
pH = 6.1





[Tris0] : [Tris] = 1 : 100
b) Range of Tris
Range of any buffer is:
From (pKa -1) to (pKa+1)
So, range of Tris is:
From (8.1 - 1) to (8.1 +1)
or from 7.1 to 9.1
Answer:
A full Moon occurs when the Moon appears as a complete circle in the sky. ... If the Moon is between the Earth and the Sun in its orbit, then the back side of the Moon is lit up and the side facing the Earth is in darkness.
Explanation:
Answer:
Nucleic acids are not considered nutrients. There are thousands of different types of molecules in a cell, but there are only four major classes of biomolecules. These are carbohydrates, lipids, proteins, and nucleic acids. Carbohydrates are compounds such as sugars and starches
Explanation:
9L!!! You take the moles of N2 in the reaction and then divide by one mole of nitrogen. Then you multiply that by the number of hydrogen moles in the reaction which in this case is 3. This gives you an answer of 3. You then take the number of hydrogen moles and multiply it by the 3L giving you 9L of hydrogen.