Question

A scientist is measuring the rate of an enzyme-catalyzed reaction that produces a red-colored product. as the solution containing the product becomes more concentrated, how do you predict absorbance (a) and transmittance (t) to change?

Answer 1

The answer would be A will increase and T will decrease.

The product of this reaction emits red light because it absorbs green and blue light. As the reaction occurs, the concentration of the product increase. This will makes absorbance of green and blue light increases and the solution will become redder.

Answer 2

Answer: Absorbance will increase; Transmittance will decrease

Explanation:

According to the Beer-Lambert law the attenuation of light depends on the material it is travelling through. The Beer-Lambert Equation is defined by A = ε l c.

A = Absorbance of light by the material

ε = molar extinction co-efficient of the material

l = path length of light

c= concentration of the material

In this case the material is the substance producing red color.

From the above equation absorbance (A)  is directly proportional concentration, then absorbance will increase with increase in concentration of the product.

Transmittance (t) is the amount of light able to be transmitted through the material. Thus absorbance and transmittance are inversely proportional and defined by the Equation: Absorbance = 2 - log (%T)

%T =  Fraction of light that is transmitted, an increase in absorbance will cause a decrease in transmittance.