The first major difference between prokaryote and eukaryote gene expression is the location. Due to lack of compartments (nucleus) prokaryotic gene expression (both transcription and translation) occurs within the cytoplasm (genetic material is located within the cytoplasm). So, transcription and translation in prokaryote occurs simultaneously. Gene expression in eukaryote occurs in both the nucleus (transcription) and cytoplasm (translation).
The steps of regulation of gene expression are also dissimilar, in prokaryotic cell, transcription is mostly the main point of regulation (eukaryote have transcription, post-transcription, translation, and post-translation regulation together with epigenetic regulation).
