1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Mrrafil [7]
3 years ago
5

Which step in the PCR process will be affected?

Biology
1 answer:
Temka [501]3 years ago
4 0

The denaturation step in the polymerase chain reaction will be affected. As a result the DNA strands will not unwind and the DNA will not become single stranded.

<u>Explanation:</u><u> </u>

Polymerase chain reaction is used to synthesize multiple copies of DNA. The technique in molecular biology uses thermal cycling which involves steps of heating and cooling. There are three important steps in PCR.

Nucleic acid denaturation is the first step. In this step the DNA is heated to about 90°c and this causes the denaturation of the DNA. Two strands of DNA get separated. The second step is annealing in which the strands are cooled to 55°c.

During annealing the primers stick to the complementary sites on the DNA to facilitate its replication. The third step is extension in which the temperature of the mixture is raised to 72°c. The tag polymerase will start working on the DNA strands and replicate them.

If the denaturation step is not performed the speration of double stranded DNA into single strands won’t take place.

You might be interested in
How does temperature change affect surface tension
qwelly [4]
All depends on climate !!
4 0
3 years ago
Secondary succession occurs in an area where the community ha been destroyed and the soil has been ______.
Triss [41]
I believe the blank space is preserved :)
7 0
3 years ago
An atom of sodium has an atomic number of 11 and a mass number of 23. Which of the following statements is correct? *
arlik [135]

Answer:

the third one is correct

explanation

because atomic number and proton &electron number is same . neutron number is solved as atomic mass minus atomic number ( N=atomic mass - atomic number)

4 0
2 years ago
Compare and contrast Rotation and revolution
Karo-lina-s [1.5K]
Rotation is the spinning of a planet, revolution is when a planet circles the sun/planet.
5 0
3 years ago
Which of the following is NOT a characteristic of triglycerides?
Harman [31]

Answer: B.

Explanation:

3 0
3 years ago
Other questions:
  • Prokaryotes that live in almost every habitat are...
    14·1 answer
  • What form of energy is used to make photosynthesis happen?A. Solar energy
    11·2 answers
  • What is the antebrachial?
    10·1 answer
  • Will give brainliest plz help
    15·1 answer
  • 3. It is a genetic code that gives us all of our physical
    12·1 answer
  • Which is one property of silt soils?
    11·2 answers
  • A lizard sitting on a rock would be considered the level of organization.
    7·1 answer
  • What is difference in scale between a human cell and a virus.
    6·1 answer
  • In pea plants the allele for axial flowers (A) is dominant to the allele for terminal flowers (a). What is the expected genotypi
    14·1 answer
  • Name three reasons that parrots are sensitive to exploitation
    7·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!