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blsea [12.9K]
3 years ago
6

2.A calibration curve requires the preparation of a set of known concentrations of CV, which are usually prepared by dieting a s

tock solution whose concentration is known. Describe how to prepare 10. mL of a 5, 10, 15, and 20 µM CV solution using a 25 µM CV stock solution.
Chemistry
1 answer:
Aleks04 [339]3 years ago
6 0

Answer:

In order to prepare 10 mL, 5 μM; <em> 2 mL of the 25 μM stock solution will be taken and diluted with water up to 10 mL mark.</em>

In order to prepare 10 mL, 10 μM; <em>4 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.</em>

In order to prepare 10 mL, 15 μM; <em>6 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.</em>

In order to prepare 10 mL, 20 μM; <em>8 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.</em>

Explanation:

Using the dilution equation:

no of moles before dilution = no of moles after dilution.

Molarity x volume (initial)= Molarity x volume (final).

In order to prepare 10 mL, 5 μM from 25 μM solution,

Final molarity = 5 μM, final volume = 10 mL, initial molarity = 25 μM, initial volume = ?

25 x initial volume = 5 x 10

Initial volume = 50/25

                       = 2 mL

<em>2 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.</em>

<em />

In order to prepare 10 mL, 10 μM from 25 μM stock,

25 x initial volume = 10 x 10

Initial volume = 100/25 = 4 mL

<em>4 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.</em>

In order to prepare 10 mL, 15 μM from 25 μM stock,

25 x initial volume = 15 x 10

initial volume = 150/25 = 6 mL

<em>6 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.</em>

In order to prepare 10 mL, 20 μM from 25 μM stock,

25 x initial volume = 20 x 10

initial volume = 200/25 = 8 mL

<em>8 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.</em>

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