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mixer [17]
3 years ago
10

1. Separation of amino acids by ion-exchange Chromatography. Mixtures of amino acids can be analyzed by first separating the mix

ture into its components through ion-exchange chromatography. Amino acids placed on a cation-exchange resin containing sulfate (-SO3-) groups flow down the column at different rates because of two factors that influence their movement (1) ionic attraction between the sulfonate residues on the column and positively charged functional groups on the amino acids, and (2) hydrophobic interactions between amino acid side chains and the strongly hydrophobic backbone of the polystyrene resin. For each pair of amino acids listed, determine which will be eluted first from the cation-exchange column by a ph 7.0 buffer.
a.) Asp and Lys

b.) Arg and Met

c.) Glu and Val

d.) Gly and Val

e.) Ser and Ala

Chemistry
1 answer:
mart [117]3 years ago
7 0

a.) Asp and Lys

Asp will elute first from the column because it has less positively charged functional groups than Lys.

 

b.) Arg and Met

Met will elute first from the column because it has less positively charged functional groups than Lys.

c.) Glu and Val

Glu will elute first from the column because it has more negativity functional groups than Lys and will be not be much retained by the -SO₃⁻ groups from the ion-exchange coloumn.

d.) Gly and Val

 Gly will elute first from the column because Lys have a longer alkyl chain which will be attracted by the strongly hydrophobic backbone for the resin.

e.) Ser and Ala

Ser will be eluted first from the column because Ala alkyl chain will be more attracted by the strongly hydrophobic backbone for the resin. Ser have an -OH group which will decrease the hydrophobicity of the alkyl chain and will not be so much retained on the column.

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san4es73 [151]

Answer:

See explanation below (Brainlist please)

Explanation:

First of all, we need to understand what is an acidic hydrogen.

An acidic hydrogen, is the atom of hydrogen which is more propense to undergo an acid base reaction, and form a stable ion or molecule in the process.

In other words, is the hydrogen that is more vulnerable to get substracted in an acid base reaction to form another compound.

Knowing this information, gives us an idea of how a molecule can be formed and which kind of compound is formed.

Now, in this question, we have 5 molecules. Each of them is either a ketone or aldehyde, so this mean that we have the carbonile group (C = O), which means that is easier to identify the acidic hydrogen. This is because the Carbonile group is an attractor group, so, it will attract the charges by inductive effect (in some cases by resonance), and the molecule is more stable.

This can be shown by drawing the enolate ion that is formed once the molecule undergo the acid base reaction. As it's an enolate form that we are looking for, then it means that the ketone or aldehyde is undergoing an electrofilic attack with a base. This base will substract the most acidic hydrogen to form a better and stable enolate. The acidic hydrogen and the enolate form can be seen in the attached picture.

a) In the case of acetaldehyde, the most acidic will be the hydrogen of carbon 2, because the hydrogen from the carbonile, once it's substracted, the charge of the carbon cannot be stabilized by resonance. Carbon 2 hydrogens, can do this job easily.

b) Propanal happens something similar to acetaldehyde, the terminal hydrogen cannot be substracted, and carbon 3, once the hydrogen is gone, the negative charge cannot be stabilized by resonance, so hydrogens of carbon 2 can do this.

c) in the case of acetone, is easier to look because we only have the C = O between two methyl group, so you can use either carbon 1 or 3 to do the job.

d) 4 heptanone the most acidic hydrogen would be carbon 3 or 5, because they are closer to the C=O and the ion can be stabilized by resonance.

e) Finally in ciclopentanone, the most acidic hydrogen would be carbon 2 or 5.

See picture for a better understanding.

Hope it helps.

7 0
3 years ago
Which hold more heat for longer period of time ? <br><br> A. Air <br> B. Water
Mandarinka [93]

Answer:

water

Explanation:

The heat capacity of a material, along with its total mass and its temperature, tell us how much thermal energy is stored in a material. ... <u>The result is that the temperature of the water cube is much more stable than the air — the water changes much more slowly; it holds onto its temperature longer.</u>

8 0
2 years ago
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Why do we study heat in a chemistry course?
Gwar [14]

Answer:

Explanation:

The human body is constantly radiating heat to its surroundings, and human comfort is closely tied to the rate of this radiation we need to know this because its part of a daily life.

8 0
3 years ago
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laila [671]
238_{U}



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5 0
3 years ago
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julia-pushkina [17]

Explanation:

Given that,

Initial volume, V₁ = 4.40 L

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As per the relation,

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(b) Again using the above relation,

P_2=\dfrac{P_1V_1}{V_2}\\\\P_2=\dfrac{2.6\times 4.4}{0.0290 }\\\\P_2=394.48\ atm

Hence, this is the required ssolution.

8 0
2 years ago
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