Answer:
The isoelectric point is that the <u>pH </u>at which the compound is in an electronically neutral form.
For diss equations<u>, p</u>lease find them in the enclosed file.
The pIs of 2 amino acids:
- Glutamate: pI = 3,2
- Histidine: pI = 7,6
Explanation:
Formula for the pI calculation: pI = (pKa1 + pKa2)/2
Given 3 pKa :
- Acid glutamic with an acid sidechain:
Use the lower 2 pKas (corresponding with 2 -COOH groups)
pKa1 = 2,19; pKa2 = 4,25; so pI = 3,2
- Histidine with 2 amino groups:
Use the higher 2 pKas ( -COOH group and -NH= group)
pKa1 = 6; pKa2 = 9,17; so pI = 7,6
