Question

A spectrophotometer measures the transmittance or the absorbance, or both, of a particular wavelength of light after it has passed through a liquid sample. Before the transmittance or absorbance of the sample is measured, a cuvette filled only with solvent, called the blank, is placed in the spectrophotometer and measured. Select the reason that, after the blank is measured, the cuvette must be placed in the spectrophotometer in the same orientation each time that it is used. O The spectrophotometer will break if the cuvette position is changed during the experiment. O The transmittance of the liquid must be measured in the same place each time. O The cuvette will only fit into the spectrophotometer in one orientation. O The transmittance of the cuvette must be measured in the same place each time.

Answer 1

Answer:

The cuvette will only fit into the spectrophotometer in one orientation.

Explanation:

There is an arrow always marked on the cuvette that indicates how to place it inside the spectrophotometer. The sides of the cuvette also have small edges that if light passes through them, spurious light can be detected due to poor positioning. The spurious light does not get through the entire sample, if we want to measure the absorbance. With a cuvette in the indicated position, the procedure is measured and its reference value is taken, and then compared with other cuvettes to be used.

Look the picture!